Cell proliferation was determined using the MTT cell viability assay kit (Beyotime Biotechnology, Shanghai, China). ASMCs were seeded onto 96-well plates at 8,000 cells/well, and after treatment, 15 μL MTT (5 mg/mL) was added to each well and incubated for 4 h. The supernatant was then removed, and 150 μL dimethyl sulfoxide (DMSO) was added to each well. Cell viability was determined by measuring the absorbance of each well at 490 nm using an ELX800 automatic microplate reader (BioTek, USA).
Mtt cell viability assay kit
Manufactured by Beyotime
Sourced in China
The MTT Cell Viability Assay kit is a colorimetric assay used to measure the metabolic activity of cells. It is based on the reduction of the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to formazan by the mitochondrial enzymes of viable cells.
Automatically generated - may contain errors
4 protocols using mtt cell viability assay kit
1
MTT Assay for Cell Proliferation
2
MTT Cell Viability Assay for HT22 Cells
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Cell viability was determined using the MTT Cell Viability Assay kit (Beyotime Institute of Biotechnology) according to the manufacturer's instructions (16 (link)). HT22 cells were seeded at a density of 3×103 cells/well into 96-well-plates and cultured with different concentrations of DMY (0, 10, 30, 100 or 300 µmol/l) for 24 h at 37°C. Cells were exposed to OGD/R prior to a 4-h incubation with 20 µl MTT solution administered to each well. DMSO was then added to each well to dissolve the formazan particles. The absorbance in each sample was then measured at a wavelength of 450 nm using a microplate reader (Thermo Fisher Scientific, Inc.). Each assay was performed in triplicate.
3
MTT Cell Viability Assay Protocol
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Cell viability was measured using the MTT Cell Viability Assay Kit (Beyotime Biotechnology, Beijing, China) on 96-well plates. After drug treatment, cells were incubated with 20 μl of MTT solution (5 mg/ml) for 4 h at 37°C, followed by replacement of the medium with 150 µl of dimethyl sulfoxide. Absorbance was read at 570 nm on an automated microplate reader (BioTek, Winooski, VT, United States).
4
MTT Assay for Cell Viability
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Cell viability was detected by MTT Cell Viability Assay kit (Beyotime Institute of Biotechnology). Cells were seeded into 96-well-plates with the density of 5 × 103 cells in ever well and cultured with different concentrations of BCP (0, 1, 5, 10, 15, 20 and 50 mM) for 24 h at 37 °C. Cells were made the OGD/R operation prior to a 4 h incubation with 20 µL MTT/well. Then, DMSO was added to ever well to dissolve the formazan particles. These plates were rocked gently for 10 min and measured the absorbance at 490 nm with a microplate reader (Bio-RAD Model 550, Hercules, CA, USA).
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