The levels of cytokines [interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), IL-12p40, IL-18, IL-1β, and high mobility group box 1 (HMGB1)], secreted by DCs, were measured by ELISA, according to manufacturer's instructions. The ELISA detection kits of IL-6, TNF-α, IL-12p40, IL-18, and IL-1β were purchased from eBioscience (San Diego, CA, USA), and the HMGB1 ELISA kit was purchased from LifeSpan BioSciences, Inc. (Seattle, Wash).
Hmgb1 elisa kit
Manufactured by LifeSpan BioSciences
Sourced in United States
The HMGB1 ELISA kit is a quantitative immunoassay designed to measure the levels of HMGB1 (High Mobility Group Box 1) protein in various sample types. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify HMGB1 in the sample.
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Lab products found in correlation
Elisa detection kits of il 6, by Thermo Fisher Scientific (2 mentions)
Onetouch glucometer, by LifeScan (2 mentions)
Triglyceride k622, by Abcam (2 mentions)
Serum alt k752, by Abcam (2 mentions)
Il 1β elisa kit, by Abcam (1 mentions)
Et 1 elisa kit, by R&D Systems (1 mentions)
Ab92516, by Abcam (1 mentions)
Envision microplate reader, by PerkinElmer (1 mentions)
Spectramax microplate reader, by Molecular Devices (1 mentions)
Enhanced atp assay kit, by Beyotime (1 mentions)
Ab79823, by Abcam (1 mentions)
Mcp 1, by MultiSciences Biotech (1 mentions)
Ifn γ, by MultiSciences Biotech (1 mentions)
7 protocols using hmgb1 elisa kit
1
Cytokine Secretion by Dendritic Cells
2
Inflammatory Factors Serum Analysis
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Serum levels of inflammatory factors NLRP3 (kit: NLRP3 ELISA kit, LifeSpan Biosciences, LS-F31954), HMGB1 (kit: HMGB1 ELISA kit, LifeSpan Biosciences, LS-F26519), endothelin-1 (ET-1, kit: ET-1 ELISA kit, R&D Systems, QET00B), IL-1β (kit: IL-1β ELISA kit, Abcam, ab46052), and TNF-α (kit: IL-1β ELISA kit, Abcam, ab181421) were determined by enzyme-linked immunosorbent assay (ELISA) using commercial kits according to the manufacturer’s instructions.
3
Metabolic Profiling in Fasted Mice
4
Investigating CRT Translocation in 4T1 Cells
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To investigate CRT translocation, 4T1 cells were incubated with PBS, free Rg3, free DOX, Rg3 + DOX, Rg3-NPs + DOX, and Rg3-PNPs + DOX for 24 h, using 0.2 µg/mL DOX and Rg3. The cells were washed by PBS and incubated with rabbit anti-calreticulin (CRT) antibody for 30 min, washed with PBS and then incubated with anti-Rabbit IgG secondary antibody [26 (link)]. After this, the cells were fixed with 4% paraformaldehyde for 10 min, then stained with DAPI at 10 µg/mL and observed under the inverted confocal microscopy. Supernatants of 4T1 cells were collected to analyze ATP and HMBG1 levels according to the manufacturer’s instructions [27 (link)]. HMGB1 ELISA kit was purchased from Life Span BioSciences, and OD value was measured with a Spectra Maxmicroplate reader (Molecular Devices, USA). An enhanced ATP assay kit was obtained from Beyotime Biotechnology (Shanghai, China). ATP luminescence was detected by EnVision microplate reader (PerkinElmer, USA).
For in vivo ICD observation, 1 × 106 4T1 cells were injected to the right breast fat pad in female BALB/c mice. Mice were treated as indicated. At the end of the experiments, the tumors were collected and examined for ICD markers (HMGB1 and CRT) by immunohistochemical analysis. Primary antibodies CRT (ab92516), HMGB1 (ab79823) were purchased from Abcam.
For in vivo ICD observation, 1 × 106 4T1 cells were injected to the right breast fat pad in female BALB/c mice. Mice were treated as indicated. At the end of the experiments, the tumors were collected and examined for ICD markers (HMGB1 and CRT) by immunohistochemical analysis. Primary antibodies CRT (ab92516), HMGB1 (ab79823) were purchased from Abcam.
5
Metabolic Profiling in Fasted Mice
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Pyruvate tolerance test (PTT) and glucose tolerance test (GTT) were performed after an overnight (16-h) fast on mice housed on aspen chip bedding. Mice were injected i.p. with 2 g/kg body weight Na-pyruvate (PTT), or 1g/kg body weight D-glucose (GTT) dissolved in sterile saline. Blood glucose levels were measured with a One-Touch glucometer (LifeScan). For fasting and re-feeding experiments, fasted mice were fed with standard chow for 4 h, retro-orbital blood was collected for serum FFA and BHBA quantification at the Mouse Phenotyping, Physiology and Metabolism Core at the University of Pennsylvania. Triglyceride (K622) and serum ALT (K752) assay kits were purchased from Biovision. HMGB1 ELISA Kit (LS-F11642–1) was purchased from LifeSpan BioSciences.
6
Cytokine Quantification in Dendritic Cells
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The level of cytokines [interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), IL-12p40, IL-18, IL-1β, and high mobility group box 1 (HMGB1)], secreted by DCs were measured by ELISA, according to the manufacturer's instructions. The ELISA detection kits of IL-6, TNF-α, IL-12p40, IL-18, and IL-1β were purchased from eBioscience (San Diego, CA, USA) and the HMGB1 ELISA kit was purchased from LifeSpan BioSciences, Inc. (Seattle, Wash).
7
Cytokine Profiling in Respiratory Conditions
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ELISA assays were used to measure the levels of cytokines (TNF-α, IL-6, IL-1β, MCP-1, IL-12p40, CCL17, CCL22, CCL24, IFN-γ, IL-4, IL-13, and HMGB1) in BALF, serum, and AM culture supernatants. The TNF-α, IL-6, IL-1β, MCP-1, IL-12p40, IFN-γ, IL-4, IL-13, and CCL22 ELISA kits were purchased from Multisciences (Hangzhou, China); the CCL17 and CCL24 ELISA kits were purchased from Rockland (San Diego, Calif); the HMGB1 ELISA kit was purchased from LifeSpan BioSciences, Inc (Seattle, Wash). All assays were performed according to the manufacturers' protocols.
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