Log In Sign up
The largest database of trusted experimental protocols

Cd4 sk3

Manufactured by BioLegend
Visit Supplier
Sourced in United States

CD4 (SK3) is a flow cytometry antibody that binds to the CD4 surface marker on T helper cells. It is a useful tool for the identification and enumeration of CD4+ T cells.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Tumor necrosis factor alpha tnf α, by Thermo Fisher Scientific (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) 7 aad, by BioLegend (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Complete rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions) Rhil 2, by R&D Systems (1 mentions) X vivo medium, by Lonza (1 mentions) Facsaria 2, by BD (1 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (1 mentions) Cd25 2a3, by BD (1 mentions) Dynabeads human t activator cd3 cd28, by Thermo Fisher Scientific (1 mentions) Cd69 fn50, by BioLegend (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Pd 1 eh12.2h7, by BioLegend (1 mentions)

Close spelling variants of this product

CD4 PE-Cy7 (SK3, (3 citations) CD4-PerCP (clone SK3, (3 citations) Anti-CD4 BV786 (clone SK3), (2 citations) CD4-BV786 (SK3, (2 citations) Anti-human CD4 (SK3, (2 citations) CD4-APC-fire750 (clone SK3 (2 citations) Anti-CD4 (clone SK3; (2 citations) FITC conjugated anti-CD4 Multiclone SK3 and SK4 (2 citations) CD4 PE-Cy7 (SK3); CD8 PerCP-Cy5.5 (RPA-T8); CCR7 FITC (G043H7); CD45RA BV421 (HI100); HLA-DR PE (L243); CD38 BV605 (HIT2) (2 citations)

4 protocols using cd4 sk3

1

Cytokine-Stimulated Dendritic Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following flow cytometry detection antibodies were purchased from the indicated manufacturers, BD Pharmingen, San Diego, CA, USA: 7AAD, CD45 (HI30), CD3 (SP34-2), CD56 (NCAM16.2), CD8 (RPA-T8), and CD4 (SK3); BioLegend, CA, USA: CD96 (6F9), CD112R, CD80, CD83, CD86, and CD14; and Elabscience, Wuhan, China: CD40 and HLA-DR. The protein levels were detected using anti-CD96, anti-p-Akt, anti-Akt, anti-p-ERK, anti-ERK, and anti-GAPDH Abs and horseradish peroxidase- (HRP-) conjugated anti-mouse/rabbit IgG (Cell Signaling Technology, Beverly, MA, USA). DCs were stimulated using interleukin- (IL-) 4, granulocyte macrophage colony-stimulating factor (GM-CSF), IL-6, tumor necrosis factor-alpha (TNF-α), and IL-1β cytokines (PeproTech, New Jersey, USA) and prostaglandin E2 (PGE2) (Selleck, Shanghai, China). X-vivo complete medium contains X-vivo medium (Lonza, Switzerland), 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), GlutaMAX (Gibco), 100 U/mL rhIL-2 (R&D, Minnesota, USA), and 100 U/mL penicillin-streptomycin solution (Gibco), and RPMI 1640 complete medium (Gibco) also consists of 10% FBS and 100 U/mL penicillin-streptomycin.
Wu F., Yang H., Xu X., Ren C., Zheng Y., Zhang H., Cai B., Qiu R., Ren W, & Quan R. (2022). CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis. BioMed Research International, 2022, 3946754.
+ Open protocol
+ Expand
2

Isolation and Purification of TREG and TRESP Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells from healthy donors were purified using Ficoll-Paque separation (Biochrom). CD4+ cells were enriched by magnetic-activated cell sorting (Miltenyi) according to manufacturer's instructions (purity>90%). For fluorescence-activated cell sorting (FACS Aria II, BD) of CD4+CD25highCD127low TREG and CD4+CD25− TRESP, cells were stained with CD4 (SK3, Biolegend), CD25 (2A3, BD), and CD127 (R34.34, Beckman Coulter). Post-FACSort analysis by flow cytometry yielded CD25+FoxP3+ TCELL purity of >95%.
Wendering D.J., Amini L., Schlickeiser S., Reinke P., Volk H.D, & Schmueck-Henneresse M. (2019). The Value of a Rapid Test of Human Regulatory T Cell Function Needs to be Revised. Frontiers in Immunology, 10, 150.
+ Open protocol
+ Expand
3

Suppression of T cell activation by Tregs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Assays were performed as described by Canavan et al. (5 (link)). Briefly, CFSE-labeled TRESP were co-cultured with autologous TREG at TRESP/TREG ratios ranging from 1:1 to 32:1. In two parallel setups, cells were either stimulated with αCD3/28-coated microbeads (Dynabeads® Human T-Activator CD3/CD28, Thermo Fisher Scientific) at a bead/cell ratio of 0.2 adjusted to the TRESP cell number per well (5 (link), 6 (link)) or adapting the ratio of 0.2 to the total cell number per well including TREG. Stimulated and unstimulated TRESP without TREG were included as controls. For the microbead titration, TRESP were cultured alone at bead/TRESP ratios ranging from 0.1 to 0.4 (mimicking the presence of TREG). αCD154 (24–31) was added at start of incubation. Cells were incubated at 37°C for 7 h. All cell cultures were performed in X-Vivo-15 medium supplemented with 10% FCS (Lonza & Biochrom) and 100 IU/ml Penicillin/Streptomycin. After harvesting, cells were stained with CD3 (OKT3), CD4 (SK3), CD137 (4B4-4), and CD69 (FN50), all Biolegend. Dead cells were excluded (LIFE/DEADTM Fixable Blue Dead Cell Stain Kit, Thermo Fisher Scientific).
Wendering D.J., Amini L., Schlickeiser S., Reinke P., Volk H.D, & Schmueck-Henneresse M. (2019). The Value of a Rapid Test of Human Regulatory T Cell Function Needs to be Revised. Frontiers in Immunology, 10, 150.
+ Open protocol
+ Expand
4

Immunophenotyping of PBMC in Ipilimumab Trial

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected at four time points: baseline (week 1) prior to administration of ipilimumab, prior to third cycle of ipilimumab (week 7), after completion of ipilimumab (week 12), and at disease progression or week 24. Blood was obtained by venipuncture and collected in acid-citrate-dextrose tubes (BD Vacutainer). Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll density gradient centrifugation (GE Healthcare), resuspended in a 90% FBS (Gemini) and 10% DMSO (Sigma-Aldrich) solution, and cryopreserved in vapor phase liquid nitrogen until batch testing.
Cellular analysis of immune cell subsets in isolated PBMCs was performed by polychromatic flow cytometry. For cell staining, thawed cells were first incubated with a Zombie viability dye (BioLegend) to detect dying cells, followed by a surface stain procedure with an antibody cocktail consisting of anti-CD3 (SK7; BD), CD4 (SK3; BioLegend), CD8 (SK1; BD), PD-1 (EH12.2H7; BioLegend), TIM-3 (7D3; BD), HLA-DR (G46–6; BD), CD56 (HCD56; BioLegend), CD19 (HIB19; BioLegend), CD16 (3G8; BD), CD11b (M1/70; BD), CD14 (M5E2; BD), and CD33 (P67.6; BioLegend). The unbound antibodies were washed out by centrifugation and stained cells were fixed with 1% paraformaldehyde prior to acquisition on an LSR Fortessa flow cytometer (BD Biosciences), and data were analyzed using Flowjo software (BD Biosciences).
Salama A.K., Palta M., Rushing C.N., Selim M.A., Linney K.N., Czito B.G., Yoo D.S., Hanks B.A., Beasley G.M., Mosca P.J., Dumbauld C., Steadman K.N., Yi J.S., Weinhold K.J., Tyler D.S., Lee W.T, & Brizel D.M. (2020). Ipilimumab and Radiation in Patients with High-risk Resected or Regionally Advanced Melanoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(5), 1287-1295.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!