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Silastic silicon tubing

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SILASTIC silicon tubing is a flexible, transparent tubing made from silicone. It is designed for laboratory and industrial applications that require a durable, inert, and temperature-resistant material.

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Lab products found in correlation

Rubber harness, by Instech (7 mentions) Methohexital sodium, by Eli Lilly (3 mentions) Stereotaxic frame, by Stoelting (1 mentions)

7 protocols using silastic silicon tubing

1

Rat Jugular Vein Catheterization for Self-Administration

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Rats were anesthetized using ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). Ketorolac (2 mg/kg, IP) was administered post-operatively and 3 days following surgery for analgesia. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein, secured with sutures, and passed subcutaneously between the shoulder blades to exit through the skin on the back. Catheter tubing was connected to a stainless-steel cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) that was worn for the duration of self-administration. The antibiotic cefazolin (100 mg/kg) was administered IV (0.1 mL) for 3 days post-surgery. Catheters were flushed with heparin (100 IU/mL; 0.1 mL) before and after each self-administration. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA), which results in a temporary loss of muscle tone.
Wilkinson C.S., Blount H.L., Davis S., Rojas G., Wu L., Murphy N.P., Schwendt M, & Knackstedt L.A. (2023). Voluntary alcohol intake alters the motivation to seek intravenous oxycodone and neuronal activation during the reinstatement of oxycodone and sucrose seeking. bioRxiv.
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2

Jugular Vein Catheterization and Stereotaxic Surgery in Rats

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Rats were anesthetized with ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). A catheter made from SILASTIC silicon tubing (ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI, United States) was implanted in the jugular vein and secured with suture thread. The catheter exited an incision on the back where it attached to a stainless-steel cannula inside a rubber harness (Instech, Plymouth Meeting, PA, United States). Rats were next placed into a stereotaxic frame (Stoelting, Wood Dale, IL, United States). A unilateral 22 gauge stainless guide cannula (Synaptech, Marquette, MI, United States) was implanted 2 mm above the NA core according to the coordinates: AP + 1.2 mm, ML ± 1.6 mm, DV −5.5 mm (Paxinos and Watson, 2006 ). Rats were administered the analgesic ketorolac (2 mg/kg, i.p.) on the day of surgery and for 3 days post-surgery. Catheter patency was maintained with heparinized saline (100 U/mL) daily and periodically verified with methohexital sodium (10 mg/mL i.v.; Eli Lilly, Indianapolis, IN, United States).
Stennett B.A, & Knackstedt L.A. (2020). A Rat Model of Cocaine-Alcohol Polysubstance Use Reveals Altered Cocaine Seeking and Glutamate Levels in the Nucleus Accumbens. Frontiers in Neuroscience, 14, 877.
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3

Intravenous Drug Self-Administration in Rats

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Ketamine (87.5 mg/ kg, i.p.) and xylazine (5 mg/kg, i.p.) were utilized as anesthetics and were administered in a volume of 1 mL/kg. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted and secured into the jugular vein with suture thread (Surgical Specialties Corp., Wyomissing, PA). The catheter tubing passed subcutaneously and exited from the back where it was connected to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) worn by the rat for the duration of the self-administration. Keterolac was administered on the day surgery and for the following three days to provide analgesia. Heparin (100 units/mL in 0.1 mL) was administered for the duration of self-administration.
Padovan-Hernandez Y, & Knackstedt L.A. (2018). Dose-dependent reduction in cocaine-induced locomotion by Clozapine-N-Oxide in rats with a history of cocaine self-administration. Neuroscience letters, 674, 132-135.
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4

Jugular Vein Catheterization in Rats

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Rats were anesthetized using ketamine (87.5 mg/kg, IP) and xylazine (5 mg/kg, IP). Ketorolac (2 mg/kg, IP) was administered post-operatively and 3 days following surgery for analgesia. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein, secured with sutures, and passed subcutaneously between the shoulder blades to exit through the skin on the back. Catheter tubing was connected to a stainless-steel cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) that was worn for the duration of self-administration. The antibiotic cefazolin (100 mg/kg) was administered IV (0.1 mL) for 3 days post-surgery. Catheters were flushed with heparin (100 IU/mL; 0.1 mL) before and after each self-administration. Catheter patency was tested periodically with methohexital sodium (10 mg/mL; Eli Lilly, Indianapolis, IN, USA), which results in a temporary loss of muscle tone.
Wilkinson C.S., Blount H.L., Davis S., Rojas G., Wu L., Murphy N.P., Schwendt M, & Knackstedt L.A. (2023). Voluntary alcohol intake alters the motivation to seek intravenous oxycodone and neuronal activation during the reinstatement of oxycodone and sucrose seeking. Scientific Reports, 13, 19174.
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5

Surgical Procedures for Rodent Self-Administration Studies

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For both experiments, rats were anesthetized with ketamine (males 87.5 mg/kg, IP; females 60 mg/kg, IP) and xylazine (males and females 5 mg/kg, IP) prior to surgery. Carprofen (1 mg/kg, SC) was administered as an analgesic for 3 days following surgery. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein and secured via suture thread. Catheters passed subcutaneously through the shoulder region and exited the back of the animal. The catheter was secured to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth, Meeting, PA, USA). This harness was worn for the duration of the self-administration portion of the study. Catheters were flushed daily with 0.2 mL of heparinized saline (100 IU/mL), and catheter patency was verified periodically via intravenous administration of methohexital sodium (10 mg/mL, IV), which produces a temporary loss of muscle tone.
Rats used for experiment 2 also underwent stereotaxic implantation of bilateral guide cannulas (Plastics One, Roanoke, VA, USA) 2 mm above the NA core (AP 1.2 mm, ML 2.5 mm, DV − 5.5 mm). Cannulas were secured using dental cement and stainless steel skull screws, and a stylet was inserted into the cannula until microinjections were administered prior to the reinstatement tests.
Logan C.N., Bechard A.R., Hamor P.U., Wu L., Schwendt M, & Knackstedt L.A. (2020). Ceftriaxone and mGlu2/3 interactions in the nucleus accumbens core affect the reinstatement of cocaine-seeking in male and female rats. Psychopharmacology, 237(7), 2007-2018.
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6

Surgical Implantation of Intravenous Catheters and Stereotactic Cannulae for Rodent Self-Administration Studies

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Ketamine (87.5 mg/ kg, i.p.) and xylazine (5 mg/kg, i.p.) were administered prior to surgery. Keterolac was administered following surgery to provide analgesia. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted and secured into the jugular vein with suture thread (black braided silk SP117), and passed subcutaneously through the shoulder blades and exited the back. The catheter tubing was connected to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth Meeting, PA, USA) worn by the rat for the duration of the self-administration.
Immediately following catheter implantation, all rats received stereotactic surgery for the implantation of cannulae aimed at the NAc. For Experiments 1, 2, and 4, bilateral guide cannulas (Plastics One, Roanoke, VA, USA) were implanted 2 mm above the NAc (AP +1.2 mm, ML +2.5 mm, DV −5.5 mm) and secured using dental cement and stainless-steel skull screws. For Experiment 3, bilateral microdialysis guide cannulae (22 gauge, Synaptech, Marquette, MI, USA) were aimed at the NAc using the same coordinates. Cefazolin (0.1 mL, 100 mg/mL) was administered for three days after surgery. Catheters were flushed with heparinized saline (0.1 mL, 100 Units/mL) prior to and following self-administration sessions.
Logan C., LaCrosse A.L, & Knackstedt L.A. (2018). Nucleus accumbens GLT-1a overexpression reduces glutamate efflux during reinstatement of cocaine-seeking but is not sufficient to attenuate reinstatement. Neuropharmacology, 135, 297-307.
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7

Surgical Procedures for Rat Addiction Research

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For both experiments, rats were anesthetized with ketamine (males 87.5 mg/kg, IP; females 60 mg/kg, IP) and xylazine (males and females 5 mg/kg, IP) prior to surgery. Carprofen (1 mg/kg, SC) was administered as an analgesic for three days following surgery. Catheters (SILASTIC silicon tubing, ID 0.51 mm, OD 0.94 mm, Dow Corning, Midland, MI) were implanted in the jugular vein and secured via suture thread. Catheters passed subcutaneously through the shoulder region and exited the back of the animal. The catheter was secured to a cannula (Plastics One, Roanoke, VA, USA) embedded in a rubber harness (Instech, Plymouth, Meeting, PA, USA). This harness was worn for the duration of the self-administration portion of the study. Catheters were flushed daily with 0.2 mL of heparinized saline (100 IU/mL) and catheter patency was verified periodically via intravenous administration of methohexital sodium (10 mg/mL, IV), which produces a temporary loss of muscle tone.
Rats used for Experiment 2 also underwent stereotaxic implantation of bilateral guide cannulas (Plastics One, Roanoke, VA, USA) 2 mm above the NA core (AP 1.2 mm, ML 2.5 mm, DV -5.5 mm). Cannulas were secured using dental cement and stainless-steel skull screws, and a stylet was inserted into the cannula until microinjections were administered prior to the reinstatement tests.
Logan C.N., Bechard A.R., Hámor P.U., Wu L., Schwendt M., & Knackstedt L.A. (2020). Ceftriaxone and mGlu2/3 interactions in the nucleus accumbens core affect the reinstatement of cocaine-seeking in male and female rats.
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