N fluorenylmethoxycarbonyl fmoc protected amino acids

N-Fluorenylmethoxycarbonyl (Fmoc)-protected amino-acids, NovaSyn-TGR resin (loading 0.18 mM/g, 0.33 mM scale synthesis), and NovaSyn-TGRA resin (loading 0.18 mM/g, 0.33 mM scale synthesis) were purchased by Merck (Darmstadt, Germany); N,N-diisopropyl-ethylamine (DIEA), N,N-dimethylformamide (DMF, peptide synthesis grade), piperidine, dimethylformamide (DMF), N-hydroxybenzotriazole (HOBt), 2-(1-H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), triisopropylsilane (TIS), cloroform, and trifluoroacetic acid (TFA), were purchased from Sigma-Aldrich (Munich, Germany). Peptide solution was prepared by dissolving lyophilized peptide in water. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was purchased from Avanti Polar Lipids (USA). Ultrapure Milli-Q water (resistivity > 18 MΩ·cm⁻¹) was used for all experiments.

N-Fluorenylmethoxycarbonyl (Fmoc)-protected amino-acids, and NovaSyn-TGR resin (loading 0.18 mM/g, 0.33 mM scale synthesis)” resin were purchased by Merck (Darmstadt, Germany); N,N-diisopropyl-ethylamine (DIEA), N,N-dimethylformamide (DMF, peptide synthesis grade), piperidine, dimethylformammide (DMF), N-hydroxybenzotriazole (HOBt), 2-(1-H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), triisopropylsilane (TIS), and trifluoroacetic acid (TFA), were purchased from Sigma-Aldrich (Munich, Germany). Peptide solution was prepared dissolving lyophilized peptide in water. Ultrapure Milli-Q water (resistivity > 18 MΩ·cm⁻¹) was used for all experiments.