H1299 cells were washed three times with DPBS and fixed with 4% paraformaldehyde (PFA) for 20 minutes, then washed three times with DPBS and permeabilized with 0.2% Triton X‐100 in DPBS for 15 minutes, and blocked with 5% BSA (bovine serum albumin) for 1 hour. Cells were then incubated with anti‐WDR79 (Bethyl Laboratories, Inc., Montgomery, TX, USA) or anti‐UHRF1 (Bethyl Laboratories, Inc., Montgomery, TX, USA) antibodies at 4°C overnight followed by a DyLight 594‐conjugated or DyLight 488‐conjugated secondary antibody (ImmunoReagents, Inc., Raleigh, NC, USA). Cells were stained with 2‐(4‐Amidinophenyl)‐6‐indolecarbamidinedihydrochloride (DAPI) (Beyotime Biotechnology, Haimen, China) for 10 minutes and washed three times with PBST; the images were acquired with a confocal microscope.
Anti wdr79
Manufactured by Fortis Life Sciences
Sourced in United States
Anti‐WDR79 is a protein-specific antibody that can be used for the detection and analysis of the WDR79 protein in biological samples. WDR79 is a protein involved in various cellular processes. The antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of the WDR79 protein.
Automatically generated - may contain errors
3 protocols using anti wdr79
1
Immunofluorescence Staining of WDR79 and UHRF1 in H1299 Cells
2
Immunostaining of Protein Complexes in Cells
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A549 and H1299 cells were fixed with 4% paraformaldehyde for 30 min, permeabilized with 0.2% Triton X-100 for 15 min, blocked with 5% bovine serum albumin, and then incubated with anti-WDR79 (Bethyl Laboratories, Inc., Montgomery, TX, USA), anti-p53, anti-MDM2 (Santa Cruz) or anti-USP7 (Bethyl Laboratories, Inc.) antibodies at 4 °C overnight, followed by a DyLight 594-conjugated or DyLight 488-conjugated secondary antibody (ImmunoReagents, Inc., Raleigh, NC, USA). Cells were stained with 2-(4-Amidinophenyl)-6-indolecarbamidinedihydrochloride (DAPI) (Beyotime Biotechnology, Haimen, China) for 10 min, and the images were acquired with a confocal-microscope.
3
Immunofluorescence Staining of WDR79 and CYCs
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Cells were fixed with 4% paraformaldehyde for 30 min. and permeabilized with 0.2% TritonX‐100 for 15 min., blocked with 5% bovine serum albumin and incubated with anti‐WDR79 (Bethyl Laboratories, Inc.) or anti‐CYCs (Sangon Biotechnology) antibodies at 4°C overnight, followed by a dylight 594‐conjugated goat anti‐rabbit IgG antibody and dylight 488‐conjugated antimouse IgG antibody (ImmunoReagents, Inc., Raleigh, NC, USA). Cells were stained with 2‐(4‐Amidinophenyl)‐6‐indolecarbamidine dihydrochloride (DAPI) (Beyotime Biotechnology, Haimen, China) for 10 min., and the images were acquired with a confocal microscope.
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