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Csb e07430m

Manufactured by Cusabio
Sourced in United States, China

The CSB-E07430m is a laboratory equipment product. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

4 protocols using csb e07430m

1

Quantifying Chemokine Inhibition by HIFPL

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The amount of chemokines, Interleukine-8 (IL-8) and Monocyte chemoattractant protein-1 (MCP-1) produced in the peritoneum lavage supernatant was quantified using ELISA kit (Cusabio CSB-E07430m and CSB-E07274m, respectively; Cusabio, College Park, MD, USA) according to the protocol from the manufacturers. The activity of HIFPL in reducing the chemokines level was compared to the solvent (DMSO) treatment and presented as percent inhibition.
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2

Cytokine and Chemokine Quantification

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Serum concentrations of inflammatory cytokines, TNF-α (MTA00B, R&D Systems , Minneapolis, MN, USA ), IL-1β (MLB00C, R&D Systems , Minneapolis, MN, USA), IL-8 (SBJ-M0010, Senbeijia Bio. Co, Nanjing, China), MMP-1 (CSB-E07417m, CUSABIO, China), and MCP-1 (CSB-E07430m, CUSABIO, China) were measured with commercial ELISA kits according to the manufacturer’s instructions. Absorbance was measured using a Multiskan Ascent plate reader (Thermo Electron Corporation, Waltham, Massachusetts, USA ).
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3

Cytokine Profiling in Mouse and Human Prostate

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Cytokine levels in mouse plasma and prostate tissue homogenate samples from immunized mice were detected using ELISA kits for CXCL9 (E-EL-M0020c, Elabscience, Wuhan, China), CXCL10 (E-EL-M0021c, Elabscience, Wuhan, China), CXCL11 (JYM1057Mo, Elisa Lab, Wuhan, China), IFN-γ (E-EL-M0048c, Elabscience, Wuhan, China), IL-6 (CSB-E04639m, Cusabio, Wuhan, China), and MCP1 (CSB-E07430m, Cusabio, Wuhan, China). Serum cytokine levels for CP/CPPS patients were assessed using ELISA kits for CXCL9 (E-EL-H6062, Elabscience, Wuhan, China), CXCL10 (E-EL-H0050c, Elabscience, Wuhan, China), and CXCL11 (E-EL-H0051c, Elabscience, Wuhan, China).
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4

Quantification of MCP-1 and Adiponectin

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Cell culture supernatants were collected and MCP-1 and adiponectin were measured by ELISA assay (cat. nos. CSB-E07430m and CSB-E07272m; CUSABIO Technology LLC) according to the manufacturer's protocol.
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