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Agarose and yeast extract

Manufactured by Helicon

Agarose is a polysaccharide derived from red algae, commonly used as a gel matrix in electrophoresis for the separation and analysis of biomolecules such as DNA, RNA, and proteins. Yeast extract is a complex mixture of water-soluble compounds obtained from the autolysis of yeast cells, commonly used as a nutrient source in microbial culture media.

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2 protocols using agarose and yeast extract

1

Cyclodextrin-Based Levofloxacin Formulation

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Methyl-β-cyclodextrin (MCD) and 2-hydroxipropyl-β-cyclodextrin (HPCD) were obtained from Sigma Aldrich (St. Louis, MI, USA), levofloxacin (Lev) from Zhejiang Kangyu Pharm Co Ltd. (Zhejiang, China). Other chemicals: organic solvents, salts and acids—production Reakhim (Moscow, Russia). Menthol and linalool were purchased from Rotichrom GC (Carl Roth GmbH + Co. Karlsruhe, Germany). Eugenol and safrole at the highest commercial quality were purchased from Acros Organics (Flanders, Belgium). Components for LB medium were bacto tryptone, agarose and yeast extract (Helicon, Moscow, Russia), NaCl (Sigma Aldrich, St. Louis, MI, USA).
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2

Antimicrobial Compound Formulation

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Chitosan oligosaccharide lactate 5 kDa (Chit5), methyl-β-cyclodextrin (MCD), baicalein, and (+)-limonene were purchased from Sigma Aldrich (St. Louis, MI, USA). Menthol and linalool were purchased from Carl Roth GmbH (Karlsruhe, Germany). Eugenol (EG) and Menthol at the highest commercial quality were purchased from Acros Organics (Geel, Belgium). Levofloxacin (LF) was purchased from Zhejiang Kangyu Pharm Co Ltd. (Hangzhou, China). Miramistin (MM) enhanced with salicylhydroxamic acid was kindly provided by the Institute of Organic Chemistry of the Russian Academy of Sciences (Dr. Krylov S.S.). Organic solvents, salts and acids were purchased from Reakhim (Moscow, Russia). Components for LB medium were bactotrypton, agarose and yeast extract (Helicon, Moscow, Russia), and NaCl (Sigma Aldrich, St. Louis, MO, USA).
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