Fast 1394

Manufactured by Olympus

Sourced in Japan

The FAST 1394 is a high-performance data acquisition and control device designed for laboratory and industrial applications. It features a FireWire (IEEE 1394) interface, allowing for fast and reliable data transfer between the device and a host computer. The FAST 1394 is capable of acquiring and controlling a wide range of sensor and instrument types, making it a versatile solution for various data collection and monitoring tasks.

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Lab products found in correlation

Image pro plus 6, by Media Cybernetics (1 mentions) Bx61 epifluorescence microscope, by Olympus (1 mentions) Vectashield antifade solution, by Vector Laboratories (1 mentions) Rhodamine conjugated anti digoxigenin antibody, by Roche (1 mentions) Bx63 epifluorescence microscope, by Olympus (1 mentions)

3 protocols using fast 1394

Characterization of Porous Polymer Films

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HIPEs were observed by optical microscopy (Olympus BX51) with a camera QICAM (FAST1394) and the software Linksys 32. The droplet size was determined using ImageJ analysis software as the average of 100 image readings. The macroporous structure of the polyHIPEs films was observed by field emission scanning electron microscopy (FESEM; Mira from TESCAN) at an accelerating voltage of 10 kV. All samples were gold-coated. The diameters of the pores were calculated by ImageJ analysis software as the average of 100 image readings.
The total pore volume (V_T) was estimated as 1/ρ_b − 1/ρ_w where ρ_b is the polyHIPE film density and ρ_w is the wall density of pores that is the density of the NC polymer (curing PDMS/Ag-Nps nanocomposite). ρ_w corresponded to the density of the flat PDMS film containing the same amount of Ag-Nps than the respective polyHIPE film. ρ_b and ρ_w were calculated by measuring the volume and weight of the polyHIPE and flat PDMS films, respectively.
X-ray diffraction (XRD) patterns of polyHIPE films were collected by using an Empyrean diffractometer (Cu Kα radiation, 0.02° step size and 30 s counting time). In addition, polyHIPEs films were analysed by energy dispersive X-ray spectroscopy (EDS) using a Bruker AXS. To verify the presence of Ag-Nps onto the porous surface of the films, EDS analysis was performed at different points of the samples.

Blancas Flores J.M., Pérez García M.G., González Contreras G., Coronado Mendoza A, & Romero Arellano V.H. (2020). Polydimethylsiloxane nanocomposite macroporous films prepared via Pickering high internal phase emulsions as effective dielectrics for enhancing the performance of triboelectric nanogenerators. RSC Advances, 11(1), 416-424.

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Chromosome Cytological Measurement and Karyotype Analysis

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Chromosome cytological measurement and karyotype analysis followed the procedure described in previous studies [17 , 18 , 20 (link)]. A Sensys CCD camera (QIMAGING, RETIGA-SRV, FAST 1394) attached to an Olympus BX61 epifluorescence microscope (Tokyo, Japan) was used for FISH image acquisition. The chromosomes and the hybridization status of the probes used were captured under different fluorochrome channels. Mitotic metaphase images were superimposed, pseudocolored and merged in the Image-Pro plus 6.5 software (Media Cybernetics). The automatically merged images were measured using the ImageJ software (National Institutes of Health, Wayne Rasband, MD, USA). The overall chromosome size was estimated by measuring the lengths of both the short and long arms of the chromosomes, excluding satellites and nucleolar organizer regions. Homologous chromosomes were identified on the basis of chromosome lengths, morphological features, and repetitive DNA FISH signals. Final images were adjusted, and the chromosomes were organized in decreasing order by the Adobe Photoshop CS6 software. Ideograms were drawn based on the measurements and FISH signals.

Deng H., Xiang S., Guo Q., Jin W., Cai Z, & Liang G. (2019). Molecular cytogenetic analysis of genome-specific repetitive elements in Citrus clementina Hort. Ex Tan. and its taxonomic implications. BMC Plant Biology, 19, 77.

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Multiprobe FISH Analysis

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FISH was performed according to published protocols [32 (link)]. Firstly, slides were hybridized with oligo librariy 1 (biotin-labeled) and oligo librariy 2 (digoxigenin-labeled). After the first round of probing and image capture, coverslips were taken off carefully and wash the slides three times in 1X PBS (phosphate-buffered saline) (5 min each). The slides were then dehydrated in an ethanol series (70, 90, and 100 %, 5 min each), denatured again in 70 % formamide at 80 °C for 2 min, dehydrated in a second ethanol series, and reprobed with the 45S rDNA (biotin-labeled) and 5S rDNA (digoxigenin-labeled) sequences simultaneously. Biotin-labeled and digoxigenin-labeled probes were detected using a fluorescein isothiocyanate (FITC)-conjugated antibiotin antibody (Vector Laboratories) and a rhodamine-conjugated antidigoxigenin antibody (Roche Diagnostics), respectively. Chromosomes were counterstained with 4,6-diamidino-2-phenylindole (DAPI) in a VectaShield antifade solution (Vector Laboratories). Images were captured digitally using a CCD camera (QIMAGING, RETIGA-SRV, FAST 1394) attached to an Olympus BX63 epifluorescence microscope. Gray-scale images were captured for each color channel and then merged. Final image adjustments were done with Adobe Photoshop (Adobe Systems).

Li K.P., Wu Y.X., Zhao H., Wang Y., Lü X.M., Wang J.M., Xu Y., Li Z.Y, & Han Y.H. (2016). Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns. BMC Evolutionary Biology, 16, 85.

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