We used six malignant pleural mesothelioma (MPM) cell lines (ACC-MESO1, ACC-MESO4, MSTO-211H, L324, N407, and K921) and a normal pleural mesothelial cell line (MeT-5A). ACC-MESO1 and ACC-MESO4 were obtained from the Riken Cell Bank (Tsukuba, Japan). MSTO-211H was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). L324, N407, and K921 cells were generated in the laboratory and kindly donated by Dr Hidetaka Uramoto, Kanazawa Medical University, Ishikawa (16 (link)). MeT-5A cells were purchased from ATCC. Human prostate cancer PC3 cells were obtained as previously described (17 (link)). Immortalized human pulmonary fibroblast (IHPF) cells (T0490) were purchased from Applied Biological Materials Inc. (Richmond, BC, Canada). MPM, MeT-5A, PC3, and IHPF cells were maintained in RPMI-1640 medium, Medium 199, DMEM (Thermo Fisher Scientific, Inc.), and Prigrow III medium (Applied Biological Materials Inc.), respectively. All cells were cultured with GlutaMAX supplement (Thermo Fisher Scientific, Inc.), 10% heat-inactivated fetal bovine serum (FBS), and 1% (v/w) penicillin/streptomycin, and were maintained at 37°C in 5% CO2.
Acc meso 1
Manufactured by RIKEN Cell Bank
Sourced in Japan
The ACC-MESO-1 is a cell culture incubator designed for the cultivation of mesenchymal stem cells. It provides a controlled environment with temperature, humidity, and gas composition regulation to support the growth and maintenance of these cell types.
Automatically generated - may contain errors
Lab products found in correlation
Acc meso 4, by RIKEN Cell Bank (5 mentions)
Msto 211h, by American Type Culture Collection (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Nci h28, by American Type Culture Collection (2 mentions)
Met 5a, by American Type Culture Collection (2 mentions)
Medium 199, by Thermo Fisher Scientific (1 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Met 5a, by Thermo Fisher Scientific (1 mentions)
Met 5a cells, by American Type Culture Collection (1 mentions)
Prigrow 3 medium, by Applied Biological Materials (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
Nci h226, by American Type Culture Collection (1 mentions)
Nci h2452, by American Type Culture Collection (1 mentions)
Ovk18, by RIKEN Cell Bank (1 mentions)
Vmrc rcw, by RIKEN Cell Bank (1 mentions)
Minimum essential medium non essential amino acids, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Heat inactivated fbs, by Thermo Fisher Scientific (1 mentions)
5 protocols using acc meso 1
1
Malignant Pleural Mesothelioma Cell Lines Protocol
2
Cell lines for cancer research
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The human mesothelioma cell lines ACC-MESO-1 and ACC-MESO-4 were purchased from the RIKEN Cell Bank (Ibaraki, Japan), JMN was a kind gift from Dr. Brenda Gerwin and others (NCI-H226, MSTO-211H, NCI-H28 and NCI-H2452) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA). The lung cancer cell lines (A549, PC9, Lu24, and WA-hT), and the HuH-7 (hepatocellular carcinoma), MKN1 (gastric cancer), OVK18 (ovarian cancer), VMRC-RCW (renal cell carcinoma) cell lines were purchased from the RIKEN Cell Bank. The MCF7 (breast cancer), KP3 (pancreatic cancer), and HCT 116 (colon cancer) cell lines were purchased from ATCC. All cells were cultured and maintained in RPMI-1640 medium (GIBCO, Grand Island, NY) supplemented with 10 % heat-inactivated fetal calf serum.
3
Mesothelial and Mesothelioma Cell Culture
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The human mesothelial cell line MeT-5A was obtained from the American Type Culture Collection (ATCC). Human mesothelioma cell lines were obtained as follows. JMN-1B was obtained from the Brigham and Women’s Hospital Cell Culture Core Facility. H2052 and H28 were obtained from the ATCC. ACC-Meso-1 and ACC-Meso-4 were obtained from the RIKEN Cell Bank. EHMES-1 and EHMES-10 were kindly provided by Dr. Hamada (Ehime University, Japan). MeT-5A was cultured in medium 199 supplemented with 3.3 nM epidermal growth factor, 400 nM hydrocortisone, 870 nM insulin, and 10% fetal bovine serum (FBS). Other cell lines were cultured in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% FBS.
4
Cell Culture Conditions for Mesothelioma Cell Lines
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MSTO-211H (biphasic), NCI-H28 (epithelioid), and NCI-H2052 (sarcomatoid) were purchased from the ATCC. ACC-MESO-1 (sarcomatoid) and ACC-MESO-4 (epithelioid) were obtained from RIKEN Cell Bank. All cells were cultured in RPMI 1640 medium (Sigma) supplemented with 2 mM l -glutamine (Gibco), 100 units/ml penicillin, 100 µg/ml streptomycin (Gibco), 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco), 1 mM sodium pyruvate (Gibco), minimum essential medium/nonessential amino acids (Gibco), and 10% heat-inactivated FBS (Gibco), in humidified CO2 incubators at 37 °C. All cells used in this study were use in the experiments within 4 weeks of culture, and were tested for mycoplasma contamination using the DAPI staining method.
5
Diverse Cell Lines for Mesothelioma Research
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Human MPM cell lines H2052, H2452, and MSTO, the human normal mesothelial cell line MET‐5A, and human non–small‐cell lung cancer cell line H1299 were obtained from American Type Culture Collection. The human MPM cell lines ACC‐MESO‐1, ACC‐MESO‐4,10 and HMMME were obtained from the RIKEN Cell Bank. The normal human epidermal keratinocyte cell line HaCaT was kindly provided by T. Yokomizo (Department of Molecular and Cellular Biochemistry, Faculty of Medicine, Juntendo University, Japan). All cell lines were expanded for at least one passage before storage at −80°C until use. Thawed HMMME, HaCaT, MET‐5A, and the other cells were resuspended in Ham's F‐12 medium, DMEM, Tissue Culture Medium‐199, and RPMI‐1640 medium, respectively. Ham's F‐12 medium and the other media were supplemented with 15% and 10% FBS, respectively. All media were supplemented with 1% penicillin–streptomycin. All cell lines were confirmed to be free of Mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza).
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