Mouse igg2b elisa quantitation set

Manufactured by Fortis Life Sciences

Sourced in United States

The Mouse IgG2b ELISA Quantitation Set is a laboratory tool used to quantify the amount of mouse IgG2b antibodies in a sample. It provides a standardized method to measure the concentration of this specific immunoglobulin type.

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Lab products found in correlation

Elisa starter accessory kit, by Fortis Life Sciences (2 mentions) Elisa for ccl3, by R&D Systems (1 mentions)

2 protocols using mouse igg2b elisa quantitation set

Tumor Cell Secretome Analysis

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Six weeks after tumour cell or PBS injection, BM plasma and serum from peripheral blood (PB) were collected from each mouse. ELISA for CCL3 (R&D Systems, Minneapolis, MN, USA) was performed on the serum samples and BM supernatants. A mouse IgG2b ELISA Quantitation Set (Bethyl Laboratories) and ELISA Starter Accessory Kit (Bethyl Laboratories) were used to determine Ig secretion according to the manufacturer’s instructions. The results were obtained by measuring the absorbance (450 nm) with a microtiter plate reader, and the Ig light chain content was determined using Curve Expert (1.4) software.

Liu L., Yu Z., Cheng H., Mao X., Sui W., Deng S., Wei X., Lv J., Du C., Xu J., Huang W., Xia S., An G., Zhou W., Ma X., Cheng T., Qiu L, & Hao M. (2020). Multiple myeloma hinders erythropoiesis and causes anaemia owing to high levels of CCL3 in the bone marrow microenvironment. Scientific Reports, 10, 20508.

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Quantitative Ig Secretion Assay

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Human Kappa ELISA Quantitation Set (Bethyl Laboratories, AL, USA), Mouse IgG2b ELISA Quantitation Set (Bethyl Laboratories, AL, USA), and ELISA Starter Accessory Kit (Bethyl Laboratories, AL, USA) were used to determine Ig secretion according to the manufacturer’s instructions. The results were measured based on the absorbance (450 nm) with a microtiter plate reader, and the content was determined using CurveExpert 1.4 software.

Zang M., Guo J., Liu L., Jin F., Feng X., An G., Qin X., Wu Y., Lei Q., Meng B., Zhu Y., Guan Y., Deng S., Hao M., Xu Y., Zou D., Wu M., Qiu L, & Zhou W. (2020). Cdc37 suppression induces plasma cell immaturation and bortezomib resistance in multiple myeloma via Xbp1s. Oncogenesis, 9(3), 31.

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