For infection experiments, we used a HSV-1 in which GFP (green fluorescent protein) was fused to a viral capsid protein (VP26) (strain KOS, Desai et Person, 1998) . A high concentration stock of HSV-1-GFP was a generous gift from Prof. Desai (Virology Laboratories, Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted June 5, 2020. ; https://doi.org/10.1101/2020.06.04.133264 doi: bioRxiv preprint 6 Poly I:C (High molecular weight), a synthetic analogue of double-stranded RNAs that binds to the TLR3 receptor, and LPS (
LPS-EB Ultrapure E.coli 0111: BA), a component of the gram-negative bacterial membrane that binds to the TLR4 receptor were purchased from Invivogen.
Human TNF𝛼 premium grade was from Miltenyi Biotec and Interferon 𝛼-2b INTRONA from Merck MSD France.
Chansard A., Dubrulle N., Mollens M.P.d., Falanga P.B., Stephen T., Hasan M., Zandbergen G.v., Aulner N., Shorte S., & David B. (2020). Unveiling inter individual variability of human fibroblast innate immune response using robust cell-based protocols.