Anti myc tag

For immunoblotting, cells were lysed using RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) (Thermo Fisher Scientific) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor followed by centrifugation at 15,000×g for 10 min at 4 °C. Protein concentration was measured using the DC Protein Assay Kit (Bio-Rad) and equal amount of proteins were analyzed by SDS-PAGE and western blot analysis using standard procedure.
For co-immunoprecipitation experiments, cells were lysed using NP40 Lysis buffer (Boston BioProducts). Equal amounts of cleared cell lysates were adjusted to the same volume and incubated with anti-Flag tag (Sigma Aldrich) or anti-Myc tag (Thermo Fisher Scientific) magnetic beads for overnight incubation at 4 °C with gentle agitation. Beads were washed four times before elution with SDS sample buffer for immunoblot analysis.
Antibodies used in this study are anti-Flag tag (Cat# F1804, Sigma Aldrich), anti-Myc tag (Cat# 2272S, CST), anti-HA tag (Cat# 3724P, CST), anti-V5 (Cat# 13202S, CST), anti-GAPDH (Cat# 8884S, CST), anti-SAV1 (Cat# 3507S, CST), anti-GFP (Cat# A-11122, Thermo Fisher), anti-Lamin A/C (Cat# 2032, CST), anti-YAP (Cat#14074, CST, anti-YAP/TAZ (Cat#8418, CST), anti p-YAP (Cat# 4911, CST) anti Histone H3 (Cat# 4499, CST). See detailed information in Supplementary Table 3.