MALS experiment was performed at 18 °C. SrBDH1 was loaded onto a Superdex 200 increase 10/300 column (GE Healthcare) that was coupled to a miniDAWN TREOS three‐angle light scattering detector (Wyatt Technology) in combination with a RefractoMax520 refractive index detector. For calculation of the molecular mass, protein concentrations were determined from the differential refractive index with a specific refractive index increment (dn/dc) of 0.185 ml g−1. Data were analyzed with the ASTRA 6.1.4.25 software (Wyatt Technology).
Refractomax520
Manufactured by Wyatt Technology
The RefractoMax520 is a laboratory instrument designed to measure the refractive index of liquids and solids. It utilizes the principle of refraction to determine the refractive index of a sample with high accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
Astra 6, by Wyatt Technology (5 mentions)
Superdex 200 increase 10 300 column, by GE Healthcare (4 mentions)
Minidawn treos three angle light scattering detector, by Wyatt Technology (3 mentions)
Hplc system, by Agilent Technologies (1 mentions)
Superose 200 increase 10 300 gl column, by Cytiva (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Superdex 75, by GE Healthcare (1 mentions)
Superdex 200 increase 10 300, by GE Healthcare (1 mentions)
6 protocols using refractomax520
1
Molecular Mass Determination of SrBDH1 Protein
2
SEC-MALS Analysis of ρ-Rof Complex
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SEC-MALS was performed in reaction buffer (20 mM Tris-HCl, pH 8.0, 120 mM KOAc, 5 mM Mg(OAc)2, 2 mM DTT). 1 mg/ml of ρ were mixed with 2 mg/ml of Rof and incubated at 32 °C for 10 min. 60 µl of the mixture were loaded on a Superose 200 increase 10/300 GL column (Cytiva) and chromatographed on HPLC system (Agilent) coupled to a miniDAWN TREOS multi-angle light scattering and a RefractoMax 520 detector system (Wyatt Technologies). Prior to measurements, a system calibration was performed using BSA (Sigma-Aldrich). ASTRA 6.1 software was used for data analysis and molecular mass determination.
3
SEC-MALS Analysis of mPRPF39 Variants
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SEC-MALS experiments were performed at 18°C with buffer containing 50 mM Tris/HCl, pH 8.4, 300 mM NaCl, 0.02% NaN3. 80 μg of mPRPF39wt or of mPRPF39 variants were loaded onto a Superdex 200 increase 10/300 column (GE Healthcare) that was coupled to a miniDAWN TREOS three-angle light scattering detector (Wyatt Technology) in combination with a RefractoMax520 refractive index detector. For calculation of the molecular mass, protein concentrations were determined from the differential refractive index with a specific refractive index increment (dn/dc) of 0.185 ml/g. Data were analyzed with the ASTRA 6.1.4.25 software (Wyatt Technology).
4
SEC-MALS Analysis of SoBDH2
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SEC-MALS experiments were performed at 18°C. SoBDH2 was loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare) coupled to a miniDAWN TREOS three-angle light-scattering detector (Wyatt Technology) in combination with a RefractoMax520 refractive-index detector. For calculation of the molecular mass, protein concentrations were determined from the differential refractive index with a specific refractive-index increment (dn/dc) of 0.185 ml−1. Data were analyzed using the ASTRA 6.1.4.25 software (Wyatt Technology).
5
SEC-MALS Analysis of Albicidin Complexes
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SEC-MALS experiments were performed at 18 °C in buffer containing 50 mM Tris (pH 7.5), 100 mM NaCl, and 0.02% NaN3. A sample of 225 µg of AlbAS or AlbAL (with and without saturating amounts of albicidin) were loaded onto Superdex 75 or Superdex 200 increase 10/300 columns (GE Healthcare) that were coupled to a miniDAWN TREOS three-angle light-scattering detector (Wyatt Technology) in combination with a RefractoMax520 refractive index detector. For calculation of the molecular mass, protein concentrations were determined from the differential refractive index with a specific refractive index increment (dn/dc) of 0.185 mL g−1. Data were analyzed with the ASTRA 6.1.4.25 software (Wyatt Technology).
6
SEC-MALS Protein Characterization
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SEC-MALS experiments were performed at 18 °C. SoBDH2 was loaded onto a Superdex 200 increase 10/300 column (GE Healthcare) that was coupled to a miniDAWN TREOS threeangle light scattering detector (Wyatt Technology) in combination with a RefractoMax520 refractive index detector. For calculation of the molecular mass, protein concentrations were determined from the differential refractive index with a specific refractive index increment (dn/dc) of 0.185 ml -1 . Data was analyzed with the ASTRA 6.1.4.25 software (Wyatt Technology).
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