Log In Sign up
The largest database of trusted experimental protocols

Anti phospho cpla2

Manufactured by Abcam
Visit Supplier

Anti-phospho-cPLA2 is a lab equipment product that detects the phosphorylated form of cytosolic phospholipase A2 (cPLA2). cPLA2 is an enzyme that plays a role in the release of arachidonic acid, a precursor to eicosanoid signaling molecules. The anti-phospho-cPLA2 product can be used to measure the activation state of cPLA2 in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti cpla2, by Cell Signaling Technology (2 mentions) Anti p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Anti nrf2, by Cell Signaling Technology (2 mentions) Anti atf4, by Cell Signaling Technology (2 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (2 mentions) Ns 398, by Merck Group (2 mentions) Anti nf kb p65, by Cell Signaling Technology (2 mentions) Anti mouse igg and anti rabbit igg secondary horseradish peroxidase conjugated antibodies, by Jackson ImmunoResearch (2 mentions) Pam3csk4, by Merck Group (2 mentions) Anti phospho p44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Anti phospho nf kb p65, by Cell Signaling Technology (2 mentions) Anti p38 mapk, by Cell Signaling Technology (2 mentions) Anti cox 2, by Abcam (2 mentions) Pge2 elisa kit, by Enzo Life Sciences (2 mentions) Anti keap1, by Cell Signaling Technology (2 mentions) Anti β actin, by Merck Group (2 mentions) Indomethacin, by Merck Group (2 mentions)

2 protocols using anti phospho cpla2

1

Molecular Mechanisms of 4-Octyl Itaconate

Check if the same lab product or an alternative is used in the 5 most similar protocols
4-Octyl itaconate was initially supplied by Professor Richard Hartley and results were later confirmed with commercially available 4-OI (Sigma Aldrich). Pam3CSK4, dimethyl fumarate, diethyl maleate, indomethacin and NS-398 (Sigma Aldrich) were also used. Antibodies used were anti-β-actin (Sigma Aldrich), anti-COX2 (Abcam), anti-phospho-cPLA2 (Ser505), anti-cPLA2, anti-NRF2, anti-KEAP1, anti-ATF4, anti-phospho-NF-kB p65 (Ser536), anti-NF-kB p65, anti-phospho-p38 MAPK (Thr180/Tyr182), anti-p38 MAPK, anti-phospho p44/42 MAPK (Erk1/2) (Thr202/Tyr 204) and anti-p44/42 MAPK (Erk1/2) (Cell Signaling). Anti-mouse IgG and anti-rabbit IgG secondary horseradish peroxidase-conjugated antibodies (Jackson Immunoresearch) were also used. A PGE2 ELISA kit was used (Enzo Life Sciences). The Silencer Select siRNAs against NRF2 (s70522), ATF4 (s62689) and Anxa1 (s69299), as well as the Silencer Select negative control, were used (ThermoFisher Scientific).
Diskin C., Zotta A., Corcoran S.E., Tyrrell V.J., Zaslona Z., O’Donnell V.B, & O’Neill L.A. (2021). 4-Octyl-itaconate and dimethyl fumarate inhibit COX2 expression and prostaglandin production in macrophages. Journal of immunology (Baltimore, Md. : 1950), 207(10), 2561-2569.
+ Open protocol
+ Expand
2

Molecular Mechanisms of 4-Octyl Itaconate

Check if the same lab product or an alternative is used in the 5 most similar protocols
4-Octyl itaconate was initially supplied by Professor Richard Hartley and results were later confirmed with commercially available 4-OI (Sigma Aldrich). Pam3CSK4, dimethyl fumarate, diethyl maleate, indomethacin and NS-398 (Sigma Aldrich) were also used. Antibodies used were anti-β-actin (Sigma Aldrich), anti-COX2 (Abcam), anti-phospho-cPLA2 (Ser505), anti-cPLA2, anti-NRF2, anti-KEAP1, anti-ATF4, anti-phospho-NF-kB p65 (Ser536), anti-NF-kB p65, anti-phospho-p38 MAPK (Thr180/Tyr182), anti-p38 MAPK, anti-phospho p44/42 MAPK (Erk1/2) (Thr202/Tyr 204) and anti-p44/42 MAPK (Erk1/2) (Cell Signaling). Anti-mouse IgG and anti-rabbit IgG secondary horseradish peroxidase-conjugated antibodies (Jackson Immunoresearch) were also used. A PGE2 ELISA kit was used (Enzo Life Sciences). The Silencer Select siRNAs against NRF2 (s70522), ATF4 (s62689) and Anxa1 (s69299), as well as the Silencer Select negative control, were used (ThermoFisher Scientific).
Diskin C., Zotta A., Corcoran S.E., Tyrrell V.J., Zaslona Z., O’Donnell V.B, & O’Neill L.A. (2021). 4-Octyl-itaconate and dimethyl fumarate inhibit COX2 expression and prostaglandin production in macrophages. Journal of immunology (Baltimore, Md. : 1950), 207(10), 2561-2569.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!