75 mm heparinized glass capillary tubes

Manufactured by DWK Life Sciences

75 mm heparinized glass capillary tubes are designed for the collection and storage of blood samples. They are made of glass and coated with heparin, an anticoagulant that prevents blood clotting. These capillary tubes are used in various clinical and research applications that require the handling of small volumes of blood.

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Lab products found in correlation

Advia 120, by Bayer (2 mentions) Ter119, by BioLegend (1 mentions) Rb6 8c5, by BioLegend (1 mentions) Rbc lysis, by BioLegend (1 mentions) M1 70, by BioLegend (1 mentions) Ra3 6b2, by BioLegend (1 mentions) Rbc lysis buffer, by BioLegend (1 mentions)

2 protocols using 75 mm heparinized glass capillary tubes

Quantifying Haematopoietic Reconstitution

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Peripheral blood was collected using 75 mm heparinized glass capillary tubes (Kimble-Chase) via retro-orbital sinus at indicated time points. To assess haematopoietic recovery following myeloablation, white blood cell, red blood cell and platelet populations were analysed using an Advia120 (Bayer Healthcare). To quantify lineage⁺ haematopoietic cells and HSC engraftment, peripheral blood was depleted of red blood cells (RBC lysis; Biolegend), stained with pre-conjugated antibodies and analysed using flow cytometry. Antibodies and dilutions used for lineage⁺ haematopoietic cell analysis: GR1 at 1:100 (RB6-8C5; Biolegend), CD11B at 1:100 (M1/70; Biolegend), B220 at 1:100 (RA3-6B2; Biolegend), CD3 at 1:50 (17A2; Biolegend), at 1:100 CD4 (GK1.5; Biolegend), and CD8 at 1:100 (53-6.7; Biolegend). Antibodies used for haematopoietic engraftment: CD45.1 at 1:100 (A20; Biolegend), CD45.2 at 1:100 (104; Biolegend), TER119 at 1:100 (TER119; Biolegend).

Poulos M.G., Ramalingam P., Gutkin M.C., Kleppe M., Ginsberg M., Crowley M.J., Elemento O., Levine R.L., Rafii S., Kitajewski J., Greenblatt M.B., Shim J.H, & Butler J.M. (2016). Endothelial-specific inhibition of NF-κB enhances functional haematopoiesis. Nature Communications, 7, 13829.

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Hematopoietic Cell Analysis via Retro-Orbital Bleeding

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Peripheral blood was collected using 75-mm heparinized glass capillary tubes (Kimble-Chase) via retro-orbital sinus bleeds into PBS (pH 7.2) containing 10 mM EDTA. WBCs, neutrophils, RBCs, and platelet counts were analyzed using an Advia120 (Bayer Healthcare). To quantify steady-state lineage⁺ hematopoietic cells and mutlilineage HSC engraftment, peripheral blood was depleted of RBCs (RBC Lysis Buffer; Biolegend) according to the manufacturer’s recommendations, stained with indicated fluorophore-conjugated antibodies, and analyzed using flow cytometry.

Ramalingam P., Poulos M.G., Gutkin M.C., Katsnelson L., Freire A.G., Lazzari E, & Butler J.M. (2020). Endothelial mTOR maintains hematopoiesis during aging. The Journal of Experimental Medicine, 217(6), e20191212.

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