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Tritc labelled goat anti mouse igg

Manufactured by Santa Cruz Biotechnology
Sourced in United States

TRITC labelled goat anti-mouse IgG is a secondary antibody conjugated with the fluorescent dye Tetramethylrhodamine (TRITC). This product is designed to detect and visualize mouse immunoglobulin G (IgG) in various immunoassays and imaging applications.

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2 protocols using tritc labelled goat anti mouse igg

1

Mapping Cx36 and MAP-2 in Hippocampus

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Paraffin-embedded sections were placed at 60 °C for 15 min, incubated in xylene at room temperature for 15 min, and then transferred sequentially into 100% EtOH, 95% EtOH, 70% EtOH, and 50% EtOH for 4 min at room temperature. Sections were rinsed in deionized water and stored in PBS. Co-localization of Cx36 with MAP-2 in hippocampus was examined by double-labelled immunofluorescence with the rabbit polyclonal antibody to rat Cx36 (1:100, Santa Cruz, USA) and the mouse polyclonal antibody against rat MAP-2 (1:100, Santa Cruz, USA). The blocking solution used was 10% normal goat serum in PBS and antibodies were diluted in 1% goat serum. The sections were incubated for 60 min with the Cx36 antibody, and the affixed antibody was detected with FITC goat anti-rabbit IgG (1:400, Santa Cruz, USA). After washing, MAP-2 antibody was incubated and detected with TRITC labelled goat anti-mouse IgG (1:400, Santa Cruz, USA). Coverslips were mounted with mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (VWR International Aps, Denmark) to identify nuclei. Co-expression was visualized using confocal microscopy (Olympus, Osaka, Japan, FV-1000).
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2

Immunofluorescence Imaging of Mitochondrial Proteins in Oocytes

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Oocytes were washed three times with phosphate-buffered saline (PBS) and then fixed in 4% paraformaldehyde in PBS at 4 °C for 1 h. Fixed cells were washed 3 times with PBST (PBS supplemented with 0.1% Tween 20) and incubated in 0.1% Triton X-100 in PBS at room temperature for 30 min. After washing with PBST, oocytes were blocked with 5% bovine serum albumin in PBS at room temperature for 1 h and transferred into diluted media containing a rabbit anti-Mfn2 antibody (1:50, a kind gift of Professor Chen Kuang-Hueih), a monoclonal anti-tubulin antibody (1:100), or a rabbit anti-p-p38 antibody (1:100) either at room temperature for 2 h or overnight at 4 °C. Finally, the labelled oocytes were washed with PBST and stained with FITC-labelled goat-anti-rabbit IgG (1:100) and TRITC-labelled goat-anti-mouse IgG (1:100) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) at room temperature for 2 h. For mitochondrial staining, oocytes were incubated for 30 min at 37 °C in M2 medium supplemented with 200 nM MitoTracker Red. The oocytes were finally stained with Hoechst 333342 after three washes in washing buffer and were mounted on glass slides for immunofluorescence microscopy. Photos were captured using a confocal laser-scanning microscope (Zeiss LSM 780, Oberkochen, Germany).
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