Fluorescein isothiocyanate fitc conjugated goat anti rabbit igg

For immunoblotting analysis, total protein extracts of coenurus and purified rTmGP50 were separated by SDS-PAGE and subsequently transferred onto polyvinylidene fluoride membranes (Boster, Wuhan, China). The remainder of the immunoblotting procedure was performed as described previously [21 (link)]. For immunolocalization studies, procedures were performed as described elsewhere [21 (link)], with modified rabbit anti-TmGP50 antibodies (1:400) and fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG (1:300; Boster).

FK506 was purchased from Fujisawa Pharmaceutical Co., Ltd (Osaka, Japan), STZ was purchased from Sigma Chemical Co (St. Louis, Mo, USA), microalbumin assay kit was purchased from Exocell Inc (Philadelphia, Pa., USA), and blood glucose assay kits were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Anti-CaN, nephrin, podocin, phosphorylated NF-κB p65 (p-p65) and iNOS polyclonal antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-macrophage monoclonal (ED-1) antibody was from Boster Biotechnology (Wuhan, China). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG as well as fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG was from Boster Biotechnology (Wuhan, China). Chemiluminescence kit was from Amersham Life Science (Little Chalfont, UK).