THP-1 monocytes and different Mφ types were collected and incubated at a density of 1 × 105 cells in 100 μl of blocking buffer (DPBS + 2.5% FBS) for 15 min at 4˚C. Cells were then incubated with phycoerythrin (PE)-conjugated mouse anti-human CD11b, allophycocyanin (APC)-conjugated mouse anti-human CD11c, APC-conjugated mouse anti-human CD80, or APC-conjugated mouse anti-human CD209 (BD Biosciences) for 30 min at 4˚C. Cells were also stained with isotype-matched control Abs which included: APC-conjugated IgG1, IgG2a, IgG2b, or PE conjugated mouse IgG1 (BD Biosciences). Following incubation, cells were washed twice with cold DPBS and finally re-suspended in 500 µl cold DPBS and run on a flow cytometer within 30 min.
Apc conjugated igg1
Manufactured by BD
APC-conjugated IgG1 is a fluorescent-labeled antibody that can be used for various applications in flow cytometry and other immunoassays. It consists of an Allophycocyanin (APC) fluorescent dye conjugated to an immunoglobulin G1 (IgG1) antibody. The APC dye allows for the detection and quantification of target cells or molecules in a sample.
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Lab products found in correlation
Igg2b, by BD (1 mentions)
Igg2a, by BD (1 mentions)
Apc conjugated mouse anti human cd11c, by BD (1 mentions)
Pe conjugated mouse igg1, by BD (1 mentions)
Pe conjugated mouse anti human cd11b, by BD (1 mentions)
Macsquant flow cytometer, by Miltenyi Biotec (1 mentions)
Apc cy7 conjugated anti cd3, by BioLegend (1 mentions)
Pe cy7 conjugated anti cd95, by BD (1 mentions)
Pe conjugated anti gr 1, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti b220, by BD (1 mentions)
Alexa fluor 647 conjugated anti gl7, by BD (1 mentions)
2 protocols using apc conjugated igg1
1
Phenotypic Characterization of Myeloid Cells
2
Flow Cytometry Analysis of Germinal Center B Cells
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Flow cytometry analysis of spleen cell suspensions was performed using the following fluorescent-labeled anti-mouse antibodies: PE-conjugated anti-B220 (BD Pharmingen #553089), PE-Cy7-conjugated anti-CD95 (BD Pharmingen #557653), Alexa Fluor 647-conjugated anti-GL7 (BD Pharmingen #561529), APC-conjugated IgG1 (BD Pharmingen #560089), APC-Cy7-conjugated anti-CD3 (Biolegend #100222), and PE-conjugated anti-Gr1 (eBioscience #12-5931-81). DAPI (4',6-diamidino-2-phenylindole) was used for the exclusion of dead cells. Cell cycle analysis of GC B cells was performed at day 10 after NP-CGG immunization, 2 h after bromodeoxyuridine (BrdU) intraperitoneal injection (2 mg), following the manufacturer’s instructions (V450-conjugated anti-BrdU, BD Horizon #560810). Data were acquired on a MACSQuant Flow Cytometer (Miltenyi Biotech) and analyzed using the FlowJo v10.1 software (TreeStar).
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