Anti glp

Protein samples were resolved by SDS-PAGE and transferred to nitrocellulose membranes. The membranes were blocked in 3% BSA buffer (10 mM Tris-HCl, pH7.4, 150 mM NaCl, 1 mM EDTA, 0.1% Tween-20 and 3% BSA). Primary antibodies used for western blotting were: anti-H3K9me1 (Cell Signaling, #14186), anti-H3K9me2 (Cell Signaling, #4658), anti-H3K9me3 (Wako Diagnostics/Chemicals, 309–34839), anti-H3K27me1 (Cell Signaling, #7693), anti-H3K27me2 (EMD Millipore, #04–821), anti-H3K27me3 (Abcam, ab6002), anti-total H3 (EMD Millipore, 06–755), anti-V5 (Abcam, ab27671), anti-phospho ERK1/2 (Cell Signaling, #4370), anti-ERK1/2 (Cell Signaling, #4695), anti-G9a (Cell Signaling, #3306), anti-GLP (Abcam, ab135487), anti-LC3B (Cell Signaling, #3868), anti-MITF (C5, in-house), anti-active β-catenin (Cell Signaling, #8814), β-catenin (Cell Signaling, #9587), anti-DKK1 (Santa Cruz, sc-374574), anti-β-actin (Santa Cruz, sc-47778), anti-α-tubilin (Sigma Aldrich, T9026), anti-Lamin A/C (Cell Signaling, #4777) and anti-Lamin B (Cell Signaling, #12586). Appropriate secondary antibodies were used in 5% skim milk/TBST buffer. Protein bands were visualized using Western lightning plus ECL (Perkin Elmer) and quantified using ImageJ software.