Dna lig4

Manufactured by Santa Cruz Biotechnology

DNA LIG4 is a laboratory equipment product used for the ligation of DNA fragments. It facilitates the joining of DNA strands through the formation of phosphodiester bonds between adjacent nucleotides. The core function of DNA LIG4 is to enable the seamless integration of DNA segments, which is a crucial step in various molecular biology techniques.

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Lab products found in correlation

Immobilon p, by Merck Group (1 mentions) Rad51, by Thermo Fisher Scientific (1 mentions) Parp1, by Thermo Fisher Scientific (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Brca1, by Santa Cruz Biotechnology (1 mentions) Xrcc4, by Santa Cruz Biotechnology (1 mentions) P p53 s15, by Cell Signaling Technology (1 mentions) P h2ax ser139, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)

2 protocols using dna lig4

Western Blot Analysis of DNA Repair Proteins

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Cell lysates were obtained by incubating a cell pellet with RIPA buffer for 30 minutes. Lysates were than resolved by SDS-PAGE. The proteins were transferred onto an Immobilon-P PVDF membrane (Millipore), which were blotted overnight with primary antibodies recognizing GAPDH, DNA LIG4 (Santa Cruz Biotechnologies), Ku70, RAD51 or PARP1 (ThermoFisher Scientific). This was followed by 1 h incubation with secondary antibodies conjugated with HRP (Anti-Mouse and Anti-Rabbit antibodies, Cell Signaling).

Czyż M., Toma M., Gajos-Michniewicz A., Majchrzak K., Hoser G., Szemraj J., Nieborowska-Skorska M., Cheng P., Gritsyuk D., Levesque M., Dummer R., Sliwinski T, & Skorski T. (2016). PARP1 inhibitor olaparib (Lynparza) exerts synthetic lethal effect against ligase 4-deficient melanomas. Oncotarget, 7(46), 75551-75560.

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Comprehensive DNA Damage Response Analysis

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The following antibodies were used for analyses: (#4588) KU70, (#9532) PARP, (#9718) p-H2AX Ser139, (#2524) p53, (#9284) p-p53 S15 from Cell Signaling Technologies (Danvers, MA), (SC9051) DNAPkc, (SC1485) KU80, (SC166488) XLF, (SC8285) XRCC4, (SC28232) DNA Lig4, (SC642) BRCA1 from Santacruz Biotech. (#07-1590) p-H2Ax Tyr 142 from EMD Millipore Corporation (Billerica, MA), (PP-H1415-00) HNF4α from Perseus proteomics, (nb100-904) 53BP1 from Novus Biolabs and (#1020) PAR from Tulips biolabs. All Alxa fluor secondary antibodies were purchased from Invitrogen, Thermo Fisher and HRP conjugated secondary antibodies were purchased from Cell Signaling Technologies (Danvers, MA).

Borude P., Bhushan B, & Apte U. (2018). DNA Damage Response Regulates Initiation of Liver Regeneration Following Acetaminophen Overdose. Gene Expression, 18(2), 115-123.

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