Cell death control

Cells were seeded 24 h before transfection in 96-well cell culture plates (Greiner Bio-One). They were transfected with a final concentration of 50 nM (for Fig. 1) or 62.5 nM miRNA mimic (Qiagen, Hilden, Germany). The Silencer Select siRNA against NFIX (siRNA ID: s9503) and HNRNPC (siRNA ID: s6721, Thermo Fisher Scientific, Darmstadt, Germany) were used for the siRNA knockdown experiments. A non-targeting siRNA (AllStars Neg. control, order number: 1027281, negative control for cell death; NT) as well as a human Cell Death control siRNA (AllStars Cell Death control, order number: 1027299, positive control for cell death; DT) (Qiagen) served as functional controls. ScreenFect A reagent (ScreenFect, Eggenstein-Leopoldshafen, Germany) was mixed 1:125 with dilution buffer. A 1 + 1 mix of ScreenFect A and miRNA mimic was incubated for 20 min at room temperature and put on the cells together with 90 µl of fresh medium^{50 (link)}. As controls, the cells were treated with 25 µM Etoposide or 0.25 µM Paclitaxel. For combination treatment with miRNAs 55 µM Carboplatin was used (all reagents from Enzo Life Sciences, Lörrach, Germany). The cells were analysed 24 h, 48 h and 72 h after transfection.