Ds fi2 300 megapixel digital camera

Samples were stained using hematoxylin solution (Mayers; Sigma Aldrich, St Louis, Missouri, United States). Immunohistochemical staining using ALP rabbit antihuman monoclonal antibody LS-B6663 (LifeSpan Biosciences Inc., Seattle, United States), rabbit anticollagen I polyclonal antibody (BIOSS, United States), and osteocalcin monoclonal antibody mouse IgG1 (Clone #190125; R&D Systems, United States) was also conducted. Microscopic observation was performed using a light microscope (Nikon H600 L, Tokyo, Japan) equipped with a DS-Fi2 300-megapixel digital camera and Nikon Image System picture editing software. Data of ALP, type 1 collagen, and osteocalcin expression were calculated using a Remelle for immunohistochemistry index scale from five different fields of view at ×400 magnification. Extension of the trabecular bone area was studied at × 200 magnification.

Following the elapse of the testing period, termination of the research subjects was undertaken. Immunohistochemical staining using a monoclonal mouse BMP-2 (Abcam, United Kingdom), Osx (Santa Cruz Biotechnology, Inc, Europe), and TRAP (Bioss Antibodies, Netherlands) antibodies was conducted. A light microscope (Nikon H600L, Tokyo, Japan) fitted with a DS-Fi2 300 MegaPixel digital camera and a Nikon Image System photo editor program was employed for the purposes of microscopic observation. The results were measured using the Remmele immunohistochemistry index scale to count the total number of osteoblast cells (both surface and mesenchymal) from five different fields of view at 400x magnification. Fresh bone was cut with a LEICA cryocut in a dark room at a temperature of between − 40°C and − 60°C. PKH-26 contains fluorescent proteins which were then observed by means of a fluorescent microscope (OLYMPUS FSX-100).
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The fresh bone to be cut must be wrapped in aluminum foil and conditioned in a dark room.

A termination process on mice was conducted after the duration of the research was achieved. The examination of the osteoblasts level, hematoxylin and eosin stained using Mayers (Sigma Aldrich, St Louis, USA) was used. Immunohistochemistry staining using monoclonal mouse TGF-β1 antibody (Novus Biologicals, USA) and monoclonal mouse Runx2 antibody (Novus Biologicals, USA) was conducted. The microscopic observation was performed using light microscope (Nikon H600L, Tokyo, Japan), equipped with DS-Fi2 300 megapixel digital camera and Nikon Image System picture editor software. Data were calculated using index scale of Remmele for immunohistochemistry and count the total of osteoblast cells (surface osteoblast and mesenchymal osteoblast) on five different fields of view in ×400 magnification.