Magnetic microbeads

Manufactured by BD

Sourced in United States

Magnetic microbeads are small, spherical particles that possess magnetic properties. These beads are typically composed of a magnetic core and a polymeric shell, which can be functionalized with various molecules or ligands. The primary function of magnetic microbeads is to enable the separation, isolation, and manipulation of target analytes or cells from complex samples through the application of a magnetic field.

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Lab products found in correlation

Anti human cd3, by BioLegend (2 mentions) Anti human cd28, by BioLegend (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Sartorius (1 mentions)

Spelling variants of this product

Rat anti-mouse CD4-conjugated magnetic microbeads (2 citations) Anti-B220 magnetic microbeads (2 citations)

3 protocols using magnetic microbeads

Isolation and Activation of T Cell Subsets

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Human CD3⁺, CD4⁺, and CD8⁺ T cells were isolated with magnetic microbeads (BD, 552593, 557766, and 557767), respectively. The purity of the enriched subset was validated by flow cytometry and was generally higher than 95% (CD3⁺, CD4⁺, and CD8⁺/CD3 T cells). Cells were activated with plate bound anti-human CD3 (5 µg/mL; BioLegend, 300314) and anti-human CD28 (1 µg/mL; BioLegend, 302923) for 48 hours in the presence of 400 U/mL IL-2. The medium was refreshed on day 2. CD3⁺, CD4⁺, and CD8⁺ T cells were cultured in RPMI-1640 medium and supplemented with 100 U/mL IL-2, and 10% FBS (Gibco, 10 270–106). Unless mentioned otherwise, CD3⁺, CD4⁺, and CD8⁺ T cells used in all the experiments were pretreated with 1α,25(OH)₂D₃ (50 nM) or vehicle two times at 1-day intervals.

Li P., Zhu X., Cao G., Wu R., Li K., Yuan W., Chen B., Sun G., Xia X., Zhang H., Wang X., Yin Z., Lu L, & Gao Y. (2022). 1α,25(OH)2D3 reverses exhaustion and enhances antitumor immunity of human cytotoxic T cells. Journal for Immunotherapy of Cancer, 10(3), e003477.

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Activation of CD8+ T Cells

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CD8⁺ T cells were isolated with magnetic microbeads (BD, 557766) from PBMCs of healthy donors (purity of CD8⁺/CD3⁺ T cells > 98%), then were stimulated with plate bound 5 μg/mL anti-human CD3 (BioLegend, 300314) and 1 μg/mL anti-human CD28 (BioLegend, 302934) in 48 wells for 48 h. CD8⁺ T cells were cultured in fresh RPMI-1640 medium supplemented with 10% FBS, 100 U/mL rhIL-2, and incubated at 37°C in a humidified atmosphere of 5% CO₂.

Zhu X., Li K., Liu G., Wu R., Zhang Y., Wang S., Xu M., Lu L, & Li P. (2023). Microbial metabolite butyrate promotes anti-PD-1 antitumor efficacy by modulating T cell receptor signaling of cytotoxic CD8 T cell. Gut Microbes, 15(2), 2249143.

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Inducing Dendritic Cell Maturation Using miR-17-5p

Cited 2 times

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Human GC cell lines (BGC-823, SGC-7901) were purchased from Chinese Academy of Sciences (Shanghai, China). All cells were maintained in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS (Biological Industries, l Beit-Haemek, Israel) and 1% penicillin and streptomycin (Thermo Fisher Scientific).
Human monocyte-derived DCs (moDCs) were isolated from buffy coat of healthy blood donors as described previously.10 (link) Bone marrow-derived dendritic cells (BMDCs) were isolated from progenitors of C57BL/6 mice.11 (link) CD11c⁺ cells at fifth day were positively isolated by magnetic microbeads (BD Biosciences, San Jose, CA, USA) with purity >90% as confirmed by flow cytometry. Experiments were performed with the approval of the Scientific Investigation Board of The Second Hospital of Hebei Medical University (Shijiazhuang, China).
Purified BMDCs were seeded onto 24-well plates, and stimulated with LPS (mature DC), untreated (immature DC [imDC]), or treated with miR-17-5p mimics/scramble control with Lipofectamine RNAIMAX (Thermo Fisher Scientific). Cells were incubated for 12 hours and then lipopolysaccharide (LPS) was added to induce maturation for another 12 hours. The efficiency of transfection was validated by RT-PCR.

Cui Z.J., Xie X.L., Qi W., Yang Y.C., Bai Y., Han J., Ding Q, & Jiang H.Q. (2019). Cell-free miR-17-5p as a diagnostic biomarker for gastric cancer inhibits dendritic cell maturation. OncoTargets and therapy, 12, 2661-2675.

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