Cell culture reagents including high glucose DMEM (Dulbecco’s Modified Eagle Medium), (β-D -arabinofuranosyl cytosine, D -glucose, ploy L -lysine, BCA protein estimation kit), and primary antibodies such as mouse monoclonal anti-β-actin (A5316), anti-Ube3a (E8655), and anti-Tuj1 (T8660) were purchased from Sigma. Fetal bovine serum (FBS), trypsin (0.25%), penicillin-streptomycin, neurobasal medium, minimum essential medium, Lipofectamine®2000, Opti-MEM, sodium pyruvate were procured from Gibco/Thermo Fisher Scientific. Mouse monoclonal anti-Ube3a was purchased from Santa Cruz Biotechnology (SC-16689) and BD Bioscience (611416). Rabbit monoclonal anti-H3(4499), anti-H3(K9) (9649), anti-H4(13919), anti-H4(K12) (2591) and anti-RAD23A; mouse monoclonal anti-HDAC1(5356) and anti-HDAC2(5113) were purchased from Cell Signaling Technology. Rabbit polyclonal anti-BDNF (NBP1-59304) was from Novus Biologicals and anti-ubiquitin (Z0458) was from DAKO. Horseradish peroxidase (HRP) conjugated and biotinylated secondary antibodies, VectaStain ABC kit, ImmPACT NovaRED HRP substrate kits were purchased from Vector laboratories. Ube3a siRNA and control siRNA were procured from Santa Cruz Biotechnology.
Anti hdac1 5356
Manufactured by Cell Signaling Technology
Anti-HDAC1(5356) is a primary antibody product from Cell Signaling Technology. It is designed to detect HDAC1 (Histone Deacetylase 1) expression in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Anti ubiquitin z0458, by Agilent Technologies (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Ploy l lysine, by Merck Group (1 mentions)
Bca protein estimation kit, by Merck Group (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
D glucose, by Merck Group (1 mentions)
Vectastain abc kit, by Vector Laboratories (1 mentions)
Myc agarose beads, by Merck Group (1 mentions)
Anti myd88 4283, by Cell Signaling Technology (1 mentions)
Mouse il 12p40 elisa kit, by R&D Systems (1 mentions)
Anti nfatc3 a303 572a, by Fortis Life Sciences (1 mentions)
Mouse ifn α elisa kit, by Thermo Fisher Scientific (1 mentions)
Cpg dna, by Merck Group (1 mentions)
On targetplus sirna, by GE Healthcare (1 mentions)
Ha agarose beads, by Merck Group (1 mentions)
Glutathione sepharose 4b beads, by GE Healthcare (1 mentions)
Anti ha antibody, by Merck Group (1 mentions)
Anti β actin, by Abcam (1 mentions)
2 protocols using anti hdac1 5356
1
Cell Culture Reagents and Antibodies
2
Immunoblot and Immunoprecipitation Protocols
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CpG DNA were purchased from Sigma-Aldrich. The ON-TARGETplus siRNA were from GE Healthcare. The following antibodies were used for immunoblot analysis and/or immunoprecipitation: anti-IRF7 (sc-74472), anti-IRF7 (sc-9083), anti-NFATC1 (sc-13033), anti-NFATC2 (sc-7296), anti-NFATC3 (sc-8321), anti-NFATC4 (sc-13036), and anti-NFAT5 (sc-13035) were from Santa Cruz Biotechnology, Inc.; anti-IRF7 (51–3300) was from Thermo Fisher Scientific; anti-MyD88 (4283), anti–NF-κB1 p105/p50, and anti-HDAC1 (5356) were from Cell Signaling Technology; and anti-NFATC3 (A303-572A) was from Bethyl Laboratories, Inc. HRP-conjugated anti-HA (ab1265), anti-Myc (ab62928), and anti-transferrin receptor antibody (ab84036) were from Abcam; anti–β-actin, anti-HA antibodies, anti-GST HRP conjugate antibody, HA–agarose beads, and Myc–agarose beads were purchased from Sigma-Aldrich. Glutathione Sepharose 4B beads were from GE Healthcare. Human IFN-α, TNF-α, and IL-6 ELISA kits were from Mabtech. The mouse IFN-α ELISA kit was from eBioscience. The mouse IL-12p40 ELISA kit was from R&D Systems.
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