The yeast strain GRX1-GFP-HIS3MX6 was purchased from Thermo Ficher Scientific. All the primers used in this study are listed in Supplementary Table 1 . To create the GRX1-GFP-tdTomato-kanR strain, a PCR fragment containing tdTomato-kanR and homologies to GFP-HIS3MX6 was amplified with primers C1 and C2 from pfa6a-tdTomato-kanR (constructed in our lab) and transformed to the GRX1-GFP-HIS3MX6 strain.
All the strains were grown in liquid YPD medium containing 20 g/L glucose (Sigma), 10 g/L peptone (Euromedex) and 10 g/L yeast extraction (Euromedex). When needed, 20 g/L agar (Euromedex) was added to make solid plates. YNB-URA− plates [20 g/L glucose (Sigma), 20 g/L agar (Euromedex), 1.71 g/L yeast nitrogen base without amino acids and nitrogen (Euromedex), 5 g/L ammonium sulfate (Sigma), and 0.77 g/L CSM-URA− (Euromedex)] or 5-FOA plate [20 g/L glucose (Sigma), 20 g/L agar (Euromedex), 1.71 g/L yeast nitrogen base without amino acids and nitrogen (Euromedex), 5 g/L ammonium sulfate (Sigma), 0.79 g/L CSM-URA− (Euromedex), and 1 g/L 5-FOA (Euromedex)] were used to select transformants.
All the strains were grown in liquid YPD medium containing 20 g/L glucose (Sigma), 10 g/L peptone (Euromedex) and 10 g/L yeast extraction (Euromedex). When needed, 20 g/L agar (Euromedex) was added to make solid plates. YNB-URA− plates [20 g/L glucose (Sigma), 20 g/L agar (Euromedex), 1.71 g/L yeast nitrogen base without amino acids and nitrogen (Euromedex), 5 g/L ammonium sulfate (Sigma), and 0.77 g/L CSM-URA− (Euromedex)] or 5-FOA plate [20 g/L glucose (Sigma), 20 g/L agar (Euromedex), 1.71 g/L yeast nitrogen base without amino acids and nitrogen (Euromedex), 5 g/L ammonium sulfate (Sigma), 0.79 g/L CSM-URA− (Euromedex), and 1 g/L 5-FOA (Euromedex)] were used to select transformants.