Library quantification was performed using the Invitrogen Quant-It broad range dsDNA quantification assay kit (Thermo Scientific Catalog: Q33130) with a 1:200 PicoGreen dilution. Following quantification, each library is normalized to a concentration of 35 ng/µL, using Tris–HCl, 10 mM, pH 8.0.
Quant it broad range dsdna quantification assay kit
Manufactured by Thermo Fisher Scientific
The Quant-It broad range dsDNA quantification assay kit is a laboratory product designed to quantify double-stranded DNA (dsDNA) samples within a broad range of concentrations. The kit provides the necessary reagents and protocols to enable accurate measurement of dsDNA levels in a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using quant it broad range dsdna quantification assay kit
1
Library Quantification and Normalization
2
Library Quantification and Normalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Library quantification was performed using the Invitrogen Quant-It broad range dsDNA quantification assay kit (Thermo Scientific Catalog: Q33130) with a 1:200 PicoGreen dilution. Following quantification, each library was normalized to a concentration of 35 ng/µL, using Tris-HCl, 10 mM, pH 8.0.
3
Sample Normalization and Mixing for Whole Genome Library
4
cfDNA and gDNA Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For cfDNA, initial DNA input was normalized to the range 25–52.5 ng in 50 uL of TE buffer (10mM Tris HCl 1mM EDTA, pH 8.0) according to picogreen quantification. For gDNA, an aliquot of gDNA (50–200ng in 50μL) was used as the input into DNA fragmentation (aka shearing). Shearing was performed acoustically using a Covaris focused-ultrasonicator, targeting 150bp fragments. Library preparation was performed using a commercially available kit provided by KAPA Biosystems (KAPA HyperPrep Kit with Library Amplification product KK8504) and IDT’s duplex UMI adapters. Unique 8-base dual index sequences embedded within the p5 and p7 primers (purchased from IDT) were added during PCR. Enzymatic clean-ups were performed using Beckman Coultier AMPure XP beads with elution volumes reduced to 30μL to maximize library concentration. Library quantification was performed using the Invitrogen Quant-It broad range dsDNA quantification assay kit (Thermo Scientific Catalog: Q33130).
5
Sample Normalization and Mixing for Whole Genome Library
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Initial sample quantification was performed using the Invitrogen Quant-It broad range dsDNA quantification assay kit (Thermo Scientific Catalog: Q33130) with a 1:200 PicoGreen dilution. Following quantification, each sample was normalized to a concentration of 10 ng/μL using a 1X Low TE pH 7.0 solution, then sample concentration was confirmed via PicoGreen. Sample mixing was then performed by combining an equal mass (ng) of each of the two samples (CHM1 & CHM13) needed to obtain enough material for the Whole Genome library preparation (500ng). The samples for creating the 4 libraries were normalized and mixed independently (Life Science Reporting Summary).
6
Library Quantification and Normalization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Library quantification was performed using the Invitrogen Quant-It broad range dsDNA quantification assay kit (Thermo Scientific Catalog: Q33130) with a 1:200 PicoGreen dilution. Following quantification, each library is normalized to a concentration of 35 ng/µL, using Tris-HCl, 10 mM, pH 8.0.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!