Pierce® BCA Protein Assay Kit was obtained from Thermo Scientific. Bax (2772), cleaved Caspase 3 (9664), SQSTM1/p62 (5114), Beclin1 (3495), p-ERK1/2 (4370), ERK1/2 (4695), p-mTOR (5536), mTOR (2983), HRP-linked anti-rabbit IgG (7074), HRP-linked anti-mouse IgG (7076) antibodies, and DAB Substrate Kit were purchased from Cell Signaling Technology. Bcl-2 (ab196495) antibody was obtained from Abcam. LC3 (M186-3) was purchased from MBL International. β-actin (BM0627) was from Boster Biological Technology. Enhanced chemiluminescence (ECL) reagent was obtained from Millipore. In Situ Cell Death Detection Kit (11684795910) was obtained from Roche Diagnostics GmbH. 3-Methyladenine (3-MA) were purchased from Selleck Chemicals.
β actin bm0627
Manufactured by Boster Bio
Sourced in China
β-actin (BM0627) is a highly conserved and ubiquitously expressed cytoskeletal protein. It is a 42 kDa protein that plays a crucial role in various cellular processes, including structural support, cell motility, and intracellular transport.
Automatically generated - may contain errors
Lab products found in correlation
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 680 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Dab substrate kit, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Selleck Chemicals (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
Gsh kit, by Nanjing Jiancheng (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Beyoecl plus p0018, by Beyotime (1 mentions)
Smad2 3 sirna sc 37238, by Santa Cruz Biotechnology (1 mentions)
Goat anti rabbit igg hrp sc 2030, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg hrp sc 2302, by Santa Cruz Biotechnology (1 mentions)
Pierce bicinchoninic acid bca protein kit, by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Affinity Biosciences (1 mentions)
4 protocols using β actin bm0627
1
Apoptosis and Autophagy Regulation
2
Camel Milk Cytokine and Antioxidant Evaluation
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CM was purchased from Xinjiang Nanshan Pasture (Urumqi, China). The milk was collected from Camelus bactrianus. After traditional pasteurization, it was transported to the laboratory via food cold chain logistics within 48 h. Cytokines TNF-α, IL-1β, IL-6, and IL-10 kits were obtained from Wuhan ColorfulGene Biological Technology Company. SOD, MDA, and GSH kits were acquired from Nanjing Jiancheng Bioengineering Institute. Antibodies for Western blot, namely, HMGB(166525-1-Ig) and TLR4 (19811-1-AP) were purchased from ProteinTech Group (Chicago, IL, USA). MyD88 (YT2928) was purchased from ImmunoWay Biotechnology Company (Plano, TX, USA). IKKβ (D30C6) was obtained from Cell Signaling Technology (Shanghai, China). NF-κB (ARG65677) was acquired from Bio-Platform Technology Company (Shanghai, China). β-actin (BM 0627) was bought from Boster Biological Technology (Wuhan, China). BeyoECL Plus (P0018) and BCA Protein Assay Kit were purchased from Beyotime Company (Shanghai, China).
3
Antibody Characterization for EMT and Autophagy
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Primary antibodies directed against AEG-1/lyric/metadherin (CST-9596), MAP-LC3 (CST-12741), TGF-β1 (CST-3711), LAMP-1 (CST-9091) and BECN1 (CST-4122) are purchased from Cell Signaling Technology, Inc. (Beverly, MA). Primary antibodies directed against E-cadherin (BS-1097), N-cadherin (BS-2224), vimentin (BS-1855), mTOR (BS-3611) and p-mTOR (BS-4706) are purchased from Bioworld Technology Co., Ltd. Primary antibody directed against β-actin (BM0627) is purchased from Boster Biological Technology, Ltd. Primary antibodies directed against AEG-1/MTDH (13860-1-AP) Snail (26183-1-AP), Twist1 (25465-1-AP), Slug (12129-1-AP) are purchased from Proteintech Technology, Inc. (Wuhan, China). Primary antibodies against Smad2/3 (sc-6202), p-Smad2/3 (Ser 423/425) (sc-11769) and Smad2/3 siRNA (sc-37238) are purchased from Santa Cruz Technology, Ltd. (Santa Cruz, CA). The secondary antibodies used are as follows: (1) anti-mouse IgG: IRDye™ 800-conjugated anti-mouse IgG (Rockland, Inc., Philadelphia, PA); (2) anti-rabbit IgG: Alexa Fluor 680 goat anti-rabbit IgG (Invitrogen, Carlsbad, CA); (3) anti-goat IgG: Alexa Fluor 680 rabbit anti-goat IgG (Invitrogen, Carlsbad, CA); (4) goat anti-mouse IgG HRP (SC-2302, Santa Cruz Biotechnology, Inc., Texas, USA); (5) goat anti-rabbit IgG HRP (SC-2030, Santa Cruz Biotechnology, Inc., Texas, USA).
4
Purification and Quantification of Rat IgG
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Total IgG was purified from SD rat serum using agarose protein G (Beyotime, Jiangsu, China). Pierce bicinchoninic acid(BCA) protein kit was used to detect IgG concentration (Thermo Fisher Scientific Inc, Waltham, US). The following chemicals were also used: DEN (Item No. N-0756, purchased from Sigma). Anti-p38 mitogen-activated protein kinase (p38 MAPK) antibody (8690T, CST); p-p38MAPK (4511T, CST); Bcl-2 (AF6139, Affinity); Bax (A00183, Affinity); β-actin (BM0627, BOSTER); and hematoxylin-eosin (HE, G1120, Sole Bauer).
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