The quantification of elements was performed using 250 mg of dried plant powder. Mineralization was carried out using 3.5 mL nitric acid (HNO3, 65%, analytical grade, Optima) and 1 mL hydrogen peroxide, and samples were incubated in a heating block digestion system (DigiPREP, SCP Sciences, Courtaboeuf, France). A gradual heating mode was used to achieve a final temperature of 100 °C (total run of 265 min). Then, ultrapure water (18.2 MΩ/cm2, Millipore Milli-Q Integral 3 system, Molsheim, France) was added to a final volume of 25 mL, and samples were filtrated to 1 μm. Elemental concentrations were determined by ICP-AES (Radial ICAP 6500 Model, Thermo Fischer Scientific, Courtaboeuf, France) or ICP-MS (X Series II Model, Thermo Fischer Scientific, Courtaboeuf, France) for REEs. Oriental basma tobacco leaves (INCT-OBTL-5, LGC Promochem, Molsheim, France) were used as certified reference material.
Radial icap 6500 model
Manufactured by Thermo Fisher Scientific
Sourced in France, United States
The Radial ICAP 6500 Model is an inductively coupled plasma (ICP) optical emission spectrometer (OES) designed for elemental analysis. It utilizes a radial plasma configuration to generate and view the plasma. The core function of this instrument is to perform quantitative and qualitative analysis of elements in a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using radial icap 6500 model
1
Quantifying Elemental Composition in Plant Samples
2
Leaf Elemental Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For each tree species, the leaves used for gas exchange measurements were washed with deionized water and dried at 70 °C for 72 h. These dried leaf samples were ground using a ball mill (Retsch MM300, Eragny, France). Mineralization was realized by acid digestion with concentrated HNO3 using a VELP DK6 heating digester (VELP Scientifica, Usmate Velate, Italy). As described by Assad et al. (2017) (link), the total elemental content (Al, B, Ca, Cd, Cu, Fe, K, Mg, Mn, Na, P, S, Si, Sr, Ti, and Zn) in the leaves from the field samples was determined using inductively coupled plasma atomic emission spectrometry (ICP-AES, Radial ICAP 6500 Model, Thermo Fischer Scientific, Inc., Pittsburgh, USA). For the pot experiment, Mn content was determined by atomic absorption spectroscopy (AAS, Agilent Technologies, Inc., Santa Clara, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!