Wildtype Xenopus laevis were bred in the animal care facility of Brown University. Following fertilization, embryos were collected and maintained in a 18 °C incubator on a 12 hr:12 hr light/dark cycle. Embryos were kept in 10% Steinberg’s solution (5.8 mM NaCl, 0.067 mM KCl, 0.034 Ca(NO3)2·4H2O, 0.083 mM MgSO4·7H2O, 5 mM HEPES, pH 7.4). Tadpole development was measured as delineated by Nieuwkoop and Faber (1956). At stage 42 (roughly 5 dpf), tadpoles were exposed to the following drug solutions: 1.25 mg/mL Fluoxetine-HCl, 2.5 mg/mL Citalopram-HBr (Sigma-Aldrich; St. Louis, MO, USA) or left in control media. Dosages were determined by creating dose–response curves, and choosing the highest dose that does not cause mortality nor morphological defects (data not shown). The drug plus Steinberg’s solution were replaced every 3 days to maintain consistent drug levels and maintain solution cleanliness for tadpole health, for a total exposure of 8–10 days, when tadpoles reach Stage 49.
Citalopram hbr
Manufactured by Merck Group
Sourced in United States
Citalopram-HBr is a chemical compound used in the manufacturing of pharmaceutical products. It serves as a key ingredient in the production of various medications. The core function of Citalopram-HBr is to provide a stable and standardized source of the active pharmaceutical ingredient, enabling the formulation of consistent and effective drug products.
Automatically generated - may contain errors
2 protocols using citalopram hbr
1
Fluoxetine and Citalopram Effects on Xenopus Tadpoles
2
Radioligand Binding Assays for Monoamine Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All SSRIs (alaproclate-HCl, citalopram-HBr, fluvoxamine-maleate, fluoxetine-HCl, norfluoxetine-HCl, paroxetine-HCl, sertraline-HCl) and other monoamine transporter inhibitors (imipramine-HCl, desipramine-HCl, clomipramine-HCl, maprotiline-HCl) were obtained from Sigma-Aldrich (St. Louis, USA). [125I]MIBG (specific activity ≈ 0.65 TBq/mmol) was purchased from Chelatec (Saint-Herblain, France). [3H]Serotonin (5-hydroxytryptamine creatinine sulfate, specific activity ≈ 3.84 TBq/mmol) was purchased from PerkinElmer.
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