Simvastatin, fluvastatin, and rosuvastatin was purchased from MCE (Shanghai, China). Abiraterone acetate was obtained from Aladdin (Shanghai, China). Polyclonal anti-β-actin, polyclonal anti-Ki67, polyclonal anti-HMGCR, polyclonal anti-SREBP1, polyclonal anti-Ki67, and anti-AR (N-20) antibodies were obtained from Santa Cruz (TX, USA). Monoclonal anti- SCAP, Monoclonal anti-CYP17A1 were obtained from Proteintech (Hubei, China), Monoclonal anti-SREBP2 were obtained from Abcam (Shanghai, China).
Polyclonal anti β actin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Polyclonal anti-β-actin is a laboratory reagent used for the detection and quantification of the beta-actin protein in biological samples. It is a mixture of antibodies that specifically bind to the beta-actin protein, which is a ubiquitous and highly conserved cytoskeletal protein found in all eukaryotic cells.
Automatically generated - may contain errors
3 protocols using polyclonal anti β actin
1
Statin Signaling Pathway Regulation
2
Antibody Characterization in Neural Cells
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The following primary antibodies were used: polyclonal anti-SVIP, monoclonal anti-NeuN (Millipore), monoclonal anti-CNS (Millipore), monoclonal anti-GFAP (Beyotime Bio, Shanghai, China), and monoclonal anti-CD11b/integrin am (OX-42). Polyclonal anti-β-actin, polyclonal anti-p53, polyclonal anti-Ki67, polyclonal anti-PSA (A67-B/E3), and anti-AR (N-20) antibodies were obtained from Santa Cruz (TX, USA). Control siRNA and siRNA targeting human SVIP have been previously described [36 ], siRNAs targeting SVIP (5′→3′): siRNA #1, GACAAAAAGAGGCTGCATC; siRNA #2, CCCTTA AGTGCAATGCTAA; siRNA #3, GACAGTTTCATAA AGCATA.
3
Antibody-Based Protein Detection
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Antibodies used in the study were from the following sources: monoclonal anti-α 1 subunit of Na/K-ATPase (Millipore, Billerica, MA), polyclonal anti-β-actin (Santa Cruz Biotechnology, Santa Cruz, CA), monoclonal Bellinzona, Switzerland), polyclonal anti-NCX3 (Cosmo Bio Ltd. Tokyo, Japan), and monoclonal pan anti-PMCA (Thermo Scientific, Rockford, IL). Immunoblotting was performed as described [6] .
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