Actin clone c4

Manufactured by MP Biomedicals

ACTIN (clone C4) is a monoclonal antibody that binds to the cytoskeletal protein actin. It is used for the detection and quantification of actin in various biological samples.

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Lab products found in correlation

Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Sf3b1, by Fortis Life Sciences (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Phospho ampkα thr172, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

β-actin (clone C4) (7 citations) Anti-actin (clone C4; (3 citations) Anti-actin (Clone C4; #08691001) antibody (2 citations)

2 protocols using actin clone c4

Immunostaining of Spliceosome Proteins

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The following antibodies were used: ACTIN (clone C4, MP Biomedicals), PRPF6 (Bethyl Laboratories), PRPF31 (clone 8E1, Abnova), PRPF4 (Abnova), BRR2 (Sigma), PRPF8 (ProteinTech), SNU114 (Novus), PRPF3 (Sigma), SF3B1 (Bethyl Laboratories), SF3B2 (Bethyl Laboratories), SNRNP70 (Sigma), Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen), and Alexa Fluor 568 donkey anti-mouse IgG (Invitrogen).

Adler A.S., McCleland M.L., Yee S., Yaylaoglu M., Hussain S., Cosino E., Quinones G., Modrusan Z., Seshagiri S., Torres E., Chopra V.S., Haley B., Zhang Z., Blackwood E.M., Singh M., Junttila M., Stephan J.P., Liu J., Pau G., Fearon E.R., Jiang Z, & Firestein R. (2014). An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. Genes & Development, 28(10), 1068-1084.

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Western Blotting of Cardiomyocyte Signaling

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For Western blotting primary cardiomyocytes were directly lysed in 200 μl Lämmli buffer per well of a 6-well plate. Lysates were sonified and boiled at 95°C for 5 min before being subjected to SDS-Page and Western blotting, For protein detection, membranes were probed with antibodies against phospho-AMPKα (Thr172, dilution 1∶1000, Cell Signaling Technologies), phospho-SAPK/JNK (Thr183/Tyr185, clone 81E11, dilution 1∶1000, Cell Signaling Technologies), phospho-PKCε (Ser729, dilution 1∶1000, Santa Cruz) and Actin (clone C4, MP biomedicals). Blots were developed with the Super Signal West Dura Extended Duration ECL reagent (Pierce/KMF Laborchemie, St. Augustin, Germany), using an appropriate peroxidase-conjugated secondary antibody. Chemiluminescence was detected with the LAS-3000 imager equipped with a CCD camera with a 16 bit resolution and band intensities were quantified using the AIDA image analyzer software (Fuji/Raytest Isotopenmessgerät GmbH). Actin was used as a loading control and all bands were normalized to it.

Goetzenich A., Kraemer S., Rossaint R., Bleilevens C., Dollo F., Siry L., Rajabi-Alampour S., Beckers C., Soppert J., Lue H., Rex S., Bernhagen J, & Stoppe C. (2014). The Role of Macrophage Migration Inhibitory Factor in Anesthetic-Induced Myocardial Preconditioning. PLoS ONE, 9(3), e92827.

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