Avanti j e centrifuge jse111330

The BGN seeds were purchased from Triotrade Johannesburg, South Africa, and used as received without sorting into the colours. The chemicals and reagents were of analytical standards. α- and β-amylase kits were from Megazyme Ltd., Ireland. All other equipment was from the Department of Food Science and Technology and Oxidative Stress Research Centre, Cape Peninsula University of Technology, Cape Town, South Africa.
The equipment and instruments used in this study were the ten trays of hot air Excalibur Food Dehydrator (Excalibur, Sacramento, CA, USA), LECO CN 628 Dumas nitrogen analyser (Leco Corp., St. Joseph, MI, USA), the centrifuge (Avanti^® J-E centrifuge JSE111330, Beckman coulter Inc., Indianapolis, IN, USA), and Thermos Scientific Multiskan plate reader spectrophotometer (Thermo Scientific, Waltham, MA, USA). Others are the pH meter (Hannah checker pH meter, Model HI1270), a water bath, and Colour Flex EZ (Model TC-P III-A, Tokyo Denshoku Co., Ltd., Tokyo, Japan). The sample treatments and analyses carried out in this section consisted of the sprout length, moisture, colour, pH, protein content, α- and β-amylase activities (Ceralpha & β-amylase enzymatic kit), total polyphenols (Folin–Ciocâlteu Reagent Assay (FCR) method), and antioxidant activities (Ferric Reducing Antioxidant Power Assay (FRAP) and 2,2-diphenyl-1-picrylhydrazyl assay (DPPH) methods).

The amylase-rich BGN malt, speciality malt, and syrups were obtained from the Department of Food Science and Technology, Cape Peninsula University, South Africa. Chemicals and reagents were of analytical standards. Alpha and beta-amylase kits were from Megazyme Ltd., Ireland. The equipment was from the Department of Food Science and Technology and Oxidative Stress Research Centre, Cape Peninsula University of Technology, Cape Town, South Africa. Equipment included the Dumas nitrogen analyser LECO CN 628 (Leco Corp., St Joseph, MI, USA). The Avanti® J-E centrifuge JSE111330 (Beckman Coulter Inc., Indianapolis, IN, USA), and Thermo Scientific MultiSkan plate reader spectrophotometer (Thermo Scientific, Waltham, MA, USA). The other equipment included the pH meter (Hanna Checker pH meter, Model HI1270), water bath, Colour Flex EZ (Model TC-P III-A, Tokyo Denshoku Co., Ltd., Tokyo, Japan), and an Excalibur Food Dehydrator (Excalibur, Sacramento, CA, USA).