R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
Myd88 knockdown constructs
Manufactured by Merck Group
Sourced in United States
MyD88 knockdown constructs are laboratory tools designed to reduce the expression of the MyD88 (Myeloid Differentiation Primary Response 88) gene. MyD88 is a key adaptor protein involved in the activation of innate immune signaling pathways. These constructs can be used in research applications to investigate the role of MyD88 in cellular processes and immune responses.
Automatically generated - may contain errors
Lab products found in correlation
Noble agar, by Merck Group (2 mentions)
Myd88, by Cell Signaling Technology (2 mentions)
Mtt reagent, by Promega (2 mentions)
Anti il 8 antibody, by Abcam (2 mentions)
Thp 1, by American Type Culture Collection (2 mentions)
Hl 60, by American Type Culture Collection (2 mentions)
α tubulin, by Cell Signaling Technology (2 mentions)
Pd08959, by Selleck Chemicals (2 mentions)
Cd11b and cd14 antibodies, by Thermo Fisher Scientific (2 mentions)
Plvx ef1alpha ires mcherry vector, by Takara Bio (2 mentions)
Hct116, by American Type Culture Collection (2 mentions)
Sp600125, by Selleck Chemicals (2 mentions)
Sb203580, by Selleck Chemicals (2 mentions)
Oci aml3, by Leibniz Institute DSMZ (2 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
Celltiter 96 aqueous one solution cell proliferation assay, by Promega (2 mentions)
2 protocols using myd88 knockdown constructs
1
Investigating TLR8 Signaling in AML
2
Investigating TLR8 Signaling in AML
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
R848, MyD88 knockdown constructs, and noble agar were from Sigma (St Louis, MO, USA). SB203580, PD08959, and SP600125 were obtained from Selleck Chem (Boston MA, USA). MTT reagent was obtained in the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega Madison WI, USA). p-p38, MyD88, α-tubulin, and actin antibodies were obtained from Cell Signaling Technologies (Beverly MA, USA). GAPDH was from Santa Cruz (Carlsbad, CA, USA). CD11b and CD14 antibodies were obtained from EBiosciences (San Diego CA, USA). Anti-Il-8 antibody was obtained from Abcam (Cambridge MA, USA). OCI-AML3 was obtained from DSMZ (Braunschweig, Germany) and 293T, HCT116, HL60, THP-1, and U937 cells were obtained from ATCC (Manassas, VA, USA). Primary AML human bone marrow cells were obtained from the CWRU Hematopoietic Stem Cell Core Facility (Cleveland, OH, USA). The cDNA for TLR8 was cloned into the pLVX-EF1alpha-IRES-mCherry vector obtained from Clontech (Mountain View, CA, USA)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!