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Anti ccr4 pe cy7

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Anti-CCR4-PE-Cy7 is a fluorochrome-conjugated monoclonal antibody that binds to the human chemokine receptor CCR4. It is designed for the detection and quantification of CCR4-expressing cells by flow cytometry.

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Lab products found in correlation

Summit 4, by Agilent Technologies (1 mentions) Live dead fixable aqua stain, by Thermo Fisher Scientific (1 mentions) Streptavidin percp cy5, by Thermo Fisher Scientific (1 mentions) Anti cxcr4 apc, by BD (1 mentions) Pe conjugated donkey polyclonal antibody to rabbit igg, by Abcam (1 mentions) Anti cxcr3 apc, by Thermo Fisher Scientific (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Anti cd16 pe, by Thermo Fisher Scientific (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Anti cxcr3 fitc, by BioLegend (1 mentions) Anti ccr6 bv605, by BioLegend (1 mentions) Anti cd3 percp cy5, by Thermo Fisher Scientific (1 mentions) Anti cd14 alexafluor647, by BioLegend (1 mentions) Cd86 fitc, by Thermo Fisher Scientific (1 mentions) Anti cd161 bv421, by BioLegend (1 mentions) Anti hla dr pe cy7, by Thermo Fisher Scientific (1 mentions) Anti cd8 apc, by Thermo Fisher Scientific (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd25 pe, by BD (1 mentions)

2 protocols using anti ccr4 pe cy7

1

Multiparameter Flow Cytometry of Immune Cells

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Mononuclear cells isolated from spleens, lymph nodes and tumours were first stained with a dead cell marker (LIVE/DEAD Fixable Aqua stain; Invitrogen) according to the manufacturer's instructions. Cells were then washed and stained for cell surface markers and chemokine receptors. Stained cells were washed, fixed and acquired on a FACS Canto II flow cytometer (BD Biosciences). Data analysis was performed using summit 4.3 software (Dako, Glostrup, Denmark). The antibodies used were as follows: anti-CD4-Pacific Blue antibody (BioLegend, San Diego, CA), goat polyclonal antibody to CCR1 (Santa Cruz Biotechnology, Santa Cruz, CA) followed with phycoerythrin (PE)-conjugated rabbit polyclonal antibody to goat IgG (Abcam, Cambridge, UK), rabbit monoclonal antibody to CCR2 (Abcam) followed with PE-conjugated donkey polyclonal antibody to rabbit IgG (Abcam), anti-CCR4-PE-Cy7 (BioLegend),anti-CCR3-Alexa Fluor 647 (BioLegend), biotin-conjugated anti-CCR5 antibodies followed with streptavidin-PerCP-Cy5.5 (both from eBioscience, San Diego, CA), anti-CXCR3-APC (eBioscience, San Diego, CA), anti-CXCR4-APC (BD Biosciences), rabbit polyclonal antibody to anti-CX3CR1 (Abcam) followed with PE-conjugated donkey polyclonal antibody to rabbit IgG (Abcam). Foxp3 expression was detected by GFP fluorescence.
Ondondo B., Colbeck E., Jones E., Smart K., Lauder S.N., Hindley J., Godkin A., Moser B., Ager A, & Gallimore A. (2015). A distinct chemokine axis does not account for enrichment of Foxp3+ CD4+ T cells in carcinogen-induced fibrosarcomas. Immunology, 145(1), 94-104.
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2

Comprehensive Immune Cell Profiling

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3x106 PBMCs were stained with Live/Dead Aqua (Life Technologies) and antibodies specific for the following human markers: anti-CD3-PercP-Cy5.5 (eBioscience), anti-CD20-Brillian Violet (BV)421 (eBioscience), anti-CD4-AlexaFluor700/PercP-Cy5.5 (eBioscience), anti-CD8-APC (eBioscience), anti-CD25-PE (BD), anti-CD14- AlexaFluor647 (BioLegend), anti-CD16-PE (eBioscience), anti-CXCR3-FITC (BioLegend), anti-CCR4-PECy-7 (BioLegend), anti-CCR6-BV605 (BioLegend) anti-CD161-BV421 (BioLegend), anti-CD45RA APCy-7 (BioLegend), anti-HLA-DR PECy7 (eBioscience), anti-CD80 CCR6-BV650 (BioLegend), CD86-FITC (Invitrogen) and CD163-APC/Cy7 (BioLegend) for 30min at 4°C. Cells were acquired on an LSR-Fortessa (BD) with consistent application settings, internal assay-control, fluorescence minus ones (FMO) control, isotype controls and/or biological control. Data was analysed using FlowJo (Tree Star, Inc. OR, USA) with gating strategies provided in the supplemental material.
Nova-Lamperti E., Romano M., Christakoudi S., Runglall M., McGregor R., Mobillo P., Kamra Y., Tsui T.L., Norris S., John S., Boardman D.A., Lechler R.I., Lombardi G, & Hernandez-Fuentes M.P. (2018). Reduced TCR-signalling contributes to impaired Th17 responses in tolerant kidney transplant recipients. Transplantation, 102(1), e10-e17.
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