Log In Sign up
The largest database of trusted experimental protocols

Cd105 fluorescein isothiocyanate fitc clone mem 226

Manufactured by Immunotools
Visit Supplier
Sourced in Germany

CD105-fluorescein isothiocyanate (FITC; clone MEM-226) is a laboratory reagent used for the detection and analysis of CD105-positive cells. It is a conjugate of the CD105 monoclonal antibody and the fluorescent dye fluorescein isothiocyanate (FITC). This reagent can be used in flow cytometry and other immunoassay applications to identify and quantify CD105-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Facscanto 2, by BD (2 mentions) Cd14 allophycocyanin apc clone m5e2, by BD (2 mentions) Cd31 pe clone wm59, by BD (2 mentions) Cd90 apc clone 5e10, by BD (2 mentions) Cd73 pe clone ad2, by BD (2 mentions) Cd45 apc clone hi30, by BD (2 mentions) Cd34 apc clone 8g12, by BD (1 mentions) Cd14 apc clone m5e2, by BD (1 mentions)

Close spelling variants of this product

CD105 (4 citations)

3 protocols using cd105 fluorescein isothiocyanate fitc clone mem 226

1

Immunophenotypic Characterization of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Surface marker expression was analysed with a FACS Canto II (BD Biosciences, NJ, USA). The following antibodies were used for immunophenotypic analysis: CD14-allophycocyanin (APC; clone M5E2), CD29-phycoerythrin (PE; clone MAR4), CD31-PE (clone WM59), CD34-APC (clone 581), CD45-APC (clone HI30), CD73-PE (clone AD2), CD90-APC (clone 5E10; all from BD Biosciences) and CD105-fluorescein isothiocyanate (FITC; clone MEM-226; ImmunoTools, Friesoythe, Germany).
Göbel C., Goetzke R., Eggermann T, & Wagner W. (2018). Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells. Scientific Reports, 8, 11676.
+ Open protocol
+ Expand
2

Immunophenotypic Surface Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunophenotypic surface marker analysis was performed on a FACS Canto II (BD, Heidelberg, Germany) upon staining with CD14 allophycocyanin (APC, clone M5E2; BD), CD29 phycoerythrin (PE, clone MAR4; BD), CD31 PE (clone WM59; BD), CD34 APC (clone 8G12; BD), CD45 APC (clone HI30; BD), CD73 PE (clone AD2; BD), CD90 APC (clone 5E10; BD), CD105 fluorescein isothiocyanate (FITC, clone MEM-226; ImmunoTools, Friesoythe, Germany).
Fernandez-Rebollo E., Mentrup B., Ebert R., Franzen J., Abagnale G., Sieben T., Ostrowska A., Hoffmann P., Roux P.F., Rath B., Goodhardt M., Lemaitre J.M., Bischof O., Jakob F, & Wagner W. (2017). Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells. Scientific Reports, 7, 5132.
+ Open protocol
+ Expand
3

Immunophenotyping of Mesenchymal Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunophenotype of MSCs and iMSCs was analyzed with a fluorescence-activated cell sorter (FACS) Canto II (BD Biosciences, Franklin Lakes, USA) after staining with the following antibodies, as described before [23 (link)]: CD29 phycoerythrin (PE; clone MAR4; BD), CD34 allophycocyanin (APC; clone 581; BD), CD31 PE (clone WM59; BD), CD45 APC (clone HI30; BD), CD105 fluorescein isothiocyanate (FITC; clone MEM-226; ImmunoTools, Friesoythe, Germany), CD90 APC (clone 5E10; BD), CD73 PE (clone AD2; BD), and CD14 APC (clone M5E2; BD).
Hollmann J., Brecht J., Goetzke R., Franzen J., Selich A., Schmidt M., Eipel M., Ostrowska A., Hapala J., Fernandez-Rebollo E., Müller-Newen G., Rothe M., Eggermann T., Zenke M, & Wagner W. (2020). Genetic barcoding reveals clonal dominance in iPSC-derived mesenchymal stromal cells. Stem Cell Research & Therapy, 11, 105.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!