Log In Sign up
The largest database of trusted experimental protocols

Prdm1 mm00476128 m1

Manufactured by Thermo Fisher Scientific
Visit Supplier

Prdm1; Mm00476128_m1 is a TaqMan® Gene Expression Assay available from Thermo Fisher Scientific. The assay is designed to detect and quantify the expression of the Prdm1 gene in mouse samples. This product provides a tool for researchers to study the expression of this gene in their laboratory experiments.

Automatically generated - may contain errors

Lab products found in correlation

Taqman gene expression assay system, by Thermo Fisher Scientific (1 mentions) Iscript select cdna synthesis kit, by Bio-Rad (1 mentions) 7300 real time pcr system, by Thermo Fisher Scientific (1 mentions) Rneasy micro kit, by Qiagen (1 mentions) Viia 7 system, by Thermo Fisher Scientific (1 mentions) Taqman fast advanced master mix, by Thermo Fisher Scientific (1 mentions) Rneasy plus kit, by Qiagen (1 mentions) Tbx21 mm00450960 m1, by Thermo Fisher Scientific (1 mentions) Iscript, by Bio-Rad (1 mentions)

Close spelling variants of this product

PRDM1

2 protocols using prdm1 mm00476128 m1

1

Gene Expression Analysis of FACS-Sorted Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from 3–10 × 104 FACS-sorted cells using the RNeasy Micro kit (QIAGEN) according to the manufacturer’s instructions. cDNA was generated using the iScript Select cDNA Synthesis kit (Bio-Rad Laboratories). Real-time PCR was performed in duplicates using the TaqMan Gene Expression Assay System on a 7300 Real-Time PCR System (Applied Biosystems). Primers for GM-CSF (Csf2; Mm01290062_m1), Blimp1 (Prdm1; Mm00476128_m1), and housekeeping gene β-actin (4352341E) were used (Applied Biosystems). Relative gene expression levels were calculated with the 2Δct method.
Weber G.F., Chousterman B.G., Hilgendorf I., Robbins C.S., Theurl I., Gerhardt L.M., Iwamoto Y., Quach T.D., Ali M., Chen J.W., Rothstein T.L., Nahrendorf M., Weissleder R, & Swirski F.K. (2014). Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis. The Journal of Experimental Medicine, 211(6), 1243-1256.
+ Open protocol
+ Expand
2

Quantitative Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was extracted with RNeasy Plus kits (Qiagen), and cDNA was made by Iscript (Bio-Rad). All of the gene expression was analyzed by quantitative real-time PCR on a ViiA7 System (Thermo Fisher Scientific) using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific) with the following primer/probe sets: Prdm1 Mm00476128_m1, Tbx21 Mm00450960_m1, Bcl6 Mm00477633_m1, and Actb (Applied Biosystems). Expression values were calculated relative to Actb detected in the same sample by duplex qPCR.
Zhu C., Dixon K.O., Newcomer K., Gu G., Xiao S., Zaghouani S., Schramm M.A., Wang C., Zhang H., Goto K., Christian E., Rangachari M., Rosenblatt-Rosen O., Okada H., Mak T., Singer M., Regev A, & Kuchroo V. (2021). Tim-3 adaptor protein Bat3 is a molecular checkpoint of T cell terminal differentiation and exhaustion. Science Advances, 7(18), eabd2710.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!