Pen strep 100

Ethanol (absolute) was obtained from Alkaloid (Skopje, Macedonia). 1,2-propanediol (p.a., >99%) was purchased from Valerus (Sofia, Bulgaria); galangin (≥95%), choline chloride (≥98%) and Nile Red from Sigma Aldrich (Switzerland); methanol (HPLC grade, ≥99.8%), D/L-lactic acid (≥88%), D (−) fructose (≥99%) and betaine (98%) from Fisher Chemical (Loughborough, UK); D/L-malic acid (>99%) from Acros Organics (Geel, Belgium); Folin-Ciocalteau reagent, dry pyridine and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) from Merck (Darmstadt, Germany); aluminium chloride anhydrous from Fluka (Buchs, Switzerland) and pinocembrin (≥95%) from Extrasynthese (Genay, France). The chemicals 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT, #M2128-1G), L-glutamine (#G7513) and Dulbecco's phosphate buffered saline (PBS, #D8537) were purchased from Merck (Sigma-Aldrich, Steinheim, Germany). HCOOH originated from Chimspektar OOD, Bulgaria. The media, sera, enzymes and antibiotics for culturing of the cell lines were delivered from Capricorn®, Germany: MEM (#MEM-A), DMEM (#DMEM-HPA), horse serum (#HOS-1A), fetal bovine serum (#FBS-HI-12A), CM-1 (#800125), Trypsin (#TRY-1B10, # TRY-2B10), Accutase® (#ACC-1B), and Pen/Strep 100 × (#PS-B).