Copper 2 sulfate pentahydrate cu2so4 5h2o

Lipase B from Candida antarctica (CAL-B) solution was purchased from Novozymes (Copenhagen, Denmark). Copper (II) sulfate pentahydrate [Cu₂SO₄ 5H₂O] and hydrogen peroxide (33%) were from Panreac (Barcelona, Spain). Sodium bicarbonate, sodium phosphate, sodium borohydride, Bisphenol A (BPA), benzoquinone, and hydroquinone were purchased from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade acetonitrile was purchased from Scharlab (Barcelona, Spain).

Lipase B from Candida antarctica (CALB) solution (Lipozyme CALB),
lipase from Thermomyces lanuginosus (TLL) solution (Lipozyme TL 100L), catalase from Aspergillus niger (CAT) solution (Catazyme), and
glucose oxidase (Gluzyme Mono 10.000 BG) (GOX) were purchased from
Novozymes (Copenhagen, Denmark). Genetically modified lipase from
((Geo)Bacillus thermocatenulatus without
native cysteines (C65S/C296S) BTL) was produced by Dr. de las Rivas
and purified following the previous report (obtaining a solution of
2.5 mg lipase/mL by Bradford assay determination).^{37 (link)} Copper(II) sulfate pentahydrate [Cu₂SO₄·5H₂O] and hydrogen peroxide (33%) were from Panreac
(Barcelona, Spain). p-Aminophenol (pNP), sodium phosphate, sodium acetate, tyrosinase (TYR) from mushroom,
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium
salt (ABTS), benzoquinone, and hydroquinone were purchased from Sigma-Aldrich
(St. Louis, MO). 3,4-Dihydroxy-l-phenylalanine (l-DOPA) was from Alfa-Aesar (MA, EEUU). 3,4-Dihydroxy-l-phenylalanine
methyl ester hydrochloride (l-DOPA methyl ester (DOPAME))
was purchased from Carbosynth (Berkshire, U.K.). Horseradish peroxidase
(HRP) was from Thermo Scientific (Madrid, Spain).