All DNA synthesis reagents were purchased from Glen Research. FNA F6 , F4 , FM6 and M6 were synthesized and purified by Sangon Biotech (Shanghai). FNA Ft6 , Ft8 and other oligonucleotides were synthesized on an ABI 3400 synthesizer (Applied Biosystems). Dabcyl CPG was used for all FAM-labeled FNA. The completed sequences were then deprotected in AMA (ammonium hydroxide/40% aqueous methylamine, 1:1) at 65 °C for 30 min and further purified by reversed-phase HPLC (ProStar; Varian) on a C-18 column using 0.1 M triethylamine acetate(TEAA) buffer (Glen Research) and acetonitrile (SigmaAldrich) as the eluents. The collected DNA products were dried and detritylated by dissolving and incubating DNA products in 200 μL of 80% acetic acid for 20 min. The detritylated DNA product was precipitated with NaCl (3 M, 25 μL) and ethanol (600 μL).
Unless otherwise noted below, all commercially available reagents and solvents were purchased from Sigma Aldrich and used without further purification. 1H NMR (TMS as the internal standard) and 19F NMR spectra (CFCl3 as the outside standard and low field positive) were recorded on a Bruker AM300 or Bruker AM400 spectrometer. 13C NMR was recorded on a Bruker AM400 spectrometer. Chemical shifts (δ) are reported in ppm, and coupling constants (J) are in Hertz (Hz).
Unless otherwise noted below, all commercially available reagents and solvents were purchased from Sigma Aldrich and used without further purification. 1H NMR (TMS as the internal standard) and 19F NMR spectra (CFCl3 as the outside standard and low field positive) were recorded on a Bruker AM300 or Bruker AM400 spectrometer. 13C NMR was recorded on a Bruker AM400 spectrometer. Chemical shifts (δ) are reported in ppm, and coupling constants (J) are in Hertz (Hz).