3 4 5 dimethythiazol 2 yl 2 5 diphenyl tetra zoliumbromide mtt dye

Manufactured by Merck Group

Sourced in Sao Tome and Principe

3-(4,5-Dimethythiazol-2-yl)-2,5-diphenyl-tetra-zoliumbromide (MTT) dye is a colorimetric assay used to measure cell metabolic activity. It is a water-soluble yellow dye that is reduced by metabolically active cells, converting it into an insoluble purple formazan product.

Automatically generated - may contain errors

Lab products found in correlation

Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Kojic acid, by Merck Group (1 mentions) Silibinin, by Yuanye Bio-Technology (1 mentions) Capsaicin, by Yuanye Bio-Technology (1 mentions) Modified griess reagent, by Merck Group (1 mentions) Ne per nuclear extraction kit, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharide lps from escherichia coli o111 b4, by Merck Group (1 mentions)

Spelling variants of this product

MTT dye (170 citations)

2 protocols using 3 4 5 dimethythiazol 2 yl 2 5 diphenyl tetra zoliumbromide mtt dye

Tyrosinase Inhibition Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mushroom tyrosinase (EC 1.14.18.1), L-3,4-dihydroxy phenylalanine (l-DOPA), and kojic acid were obtained from Sigma (St. Louis, MO, United States). Dimethyl sulfoxide (DMSO) of molecular biological grade was purchased from J&K Chemical Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM) were obtained from Gibco (Grand Island, NY, United States). The 3-(4,5-Dimethythiazol-2-yl)-2,5-diphenyl-tetra-zoliumbromide (MTT) dye was supplied by Sigma-Aldrich (St. Louis, MO, United States). All other reagents were of analytical grade.

Hu Y.G., Gao Z.P., Zheng Y.Y., Hu C.M., Lin J., Wu X.Z., Zhang X., Zhou Y.S., Xiong Z, & Zhu D.Y. (2022). Synthesis and Biological Activity Evaluation of 2-Cyanopyrrole Derivatives as Potential Tyrosinase Inhibitors. Frontiers in Chemistry, 10, 914944.

+ Open protocol

+ Expand

Anti-inflammatory Effects of Silibinin and Capsaicin

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silibinin (purity ≥98% by HPLC) and capsaicin (purity ≥98% by HPLC) were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM) were purchased from Gibco (Grand Island, NY, United States). Lipopolysaccharide (LPS, from Escherichia coli O111:B4) and 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) dye were obtained from Sigma-Aldrich (St. Louis, MO, United States). Antibodies against COX-2 (12282S), p-JNK (9251S), p-ERK (9101S), ERK (9102S), p-p38 (9211S), p38 (9212S), and p65 (8242S) were purchased from Cell Signaling Technology (Danvers, MA, United States). The IL-6 (mouse) ELISA Kit and murine TNF-α Pre-Coated ELISA kit were purchased from Cayman Chemical Co., Ltd. (Ann Arbor, MI, United States). The Griess reagent (modified) was obtained from Sigma-Aldrich. NE-PER Nuclear Extraction Kit was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, United States).

Zheng Y., Chen J., Wu X., Zhang X., Hu C., Kang Y., Lin J., Li J., Huang Y., Zhang X, & Li C. (2022). Enhanced Anti-Inflammatory Effects of Silibinin and Capsaicin Combination in Lipopolysaccharide-Induced RAW264.7 Cells by Inhibiting NF-κB and MAPK Activation. Frontiers in Chemistry, 10, 934541.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!