CD4 cell count was measured by flow cytometry (FACSCantoII, Becton Dickinson, Franklin Lakes, NJ, USA), and plasma HIV load (HIV RNA) was determined using a standardized reverse transcriptase-polymerase chain reaction assay (Cobas Amplicor HIV-1 Monitor Test, version 1.5, Ultrasensitive specimen preparation, Roche Diagnostic Systems Inc., Branchburg, NJ, USA). The lower limit of detection in plasma was 50 HIV-1 RNA copies/mL. HCV infection was detected by measuring antibodies to HCV in serum samples by using ELISA (Ortho HCV 3.0 ELISA test, Ortho Clinical Diagnostic, Amersham, Buckinghamshire, UK) and an immunoblot assay (SIA, Chiron RIBA, Chiron Corporation, Emeryville, CA, USA). HBV infection was detected by measuring the hepatitis B surface antigen level using an enzyme-linked fluorescent assay (Biomerieux, Lyon, France). Serum TSH level was measured by a one-step radioimmunometric assay (IRMA-mat, Byk-Sangtec Diagnostica, Dietzenbach, Germany), and serum FT3 and FT4 levels were measured using radioimmunological assays (Amerlex MAB, Ortho Clinical Diagnostics, Milan, Italy).
Enzyme linked fluorescent assay
Manufactured by bioMérieux
Sourced in France
The Enzyme Linked Fluorescent Assay (ELFA) is a diagnostic tool used to detect and quantify specific analytes in a sample. It utilizes an enzyme-labeled antibody that binds to the target analyte, and the resulting enzyme-substrate reaction produces a fluorescent signal, which is measured to determine the analyte's concentration.
Automatically generated - may contain errors
Lab products found in correlation
Facscanto 2, by BD (1 mentions)
Cobas amplicor hiv 1 monitor test, by Roche (1 mentions)
Amh gen 2 kit, by Beckman Coulter (1 mentions)
Architect i system equipment, by Abbott (1 mentions)
Alere determine hbsag test, by Abbott (1 mentions)
Architect hbsag qualitative 2 assay, by Abbott (1 mentions)
Vidas hbs ag ultra test, by bioMérieux (1 mentions)
6 protocols using enzyme linked fluorescent assay
1
Comprehensive Viral and Endocrine Biomarker Assessment
2
Measuring Plasma sCD14 and D-dimer
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Cryopreserved plasma was processed for sCD14 measurements in duplicate using a commercially available ELISA assay (R&D Systems, Minneapolis, MN), and analyzed according to manufacturer's recommended procedure. D-dimer levels were quantified with an enzyme linked fluorescent assay (BioMérieux Inc., Durham, North Carolina, USA).
3
COVID-19 Diagnostic Laboratory Protocol
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Specimens analyzed included mainly blood and respiratory samples. Laboratory tests included complete blood cell count, liver function tests, renal function tests, ferritin levels, serum lactate dehydrogenase (LDH) levels and D-dimer levels. Serum D-dimer levels were established using the ELFA technique (Enzyme Linked Fluorescent Assay) (BioMérieux). Chest X-rays and chest CT scans were performed when appropriate. To confirm the diagnosis of COVID-19, Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for SARS-CoV-2 in a respiratory sample was used. Patients with a positive test for SARS-CoV-2 antigen in a nasopharyngeal sample could also be included. ABBOTT's COVID-19 Ag Rapid Test was used.
4
Menstrual Cycle Serum Analysis for AMH, FSH, and Estradiol
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Fresh blood samples were collected on the 2 nd or 3 rd day of the menstrual cycle. Samples were centrifuged at 2000 g for 10 min, aliquoted in a cryopreservation tube and serum samples were stored at -80ºC until AMH, FSH and estradiol measurements were taken.
Basal FSH and estradiol levels were measured by a competition method with a final fluorescent detection (ELFA, Enzyme Linked Fluorescent Assay, Mini-Vidas-BioMerieux, Hazelwood, and Missouri).
AMH measurement was performed in the same laboratory using the same assay by one operator. The AMH serum levels were measured using an enzyme-linked immunosorbent assay (ELISA) using the AMH Gen II kit by Beckman Coulter, Inc (Brea, CA, USA).The measurement was realized by automated Labotech (Alka Technology®). The reading was performed at a wavelength at 450 nm. To calculate the AMH value, we used the point to point method. Values are presented in concentrations of ng/ml (convert to SI units: 1 ng/ ml = 7,14pM). The analytic sensibility was 0.008 ng/ml.
Basal FSH and estradiol levels were measured by a competition method with a final fluorescent detection (ELFA, Enzyme Linked Fluorescent Assay, Mini-Vidas-BioMerieux, Hazelwood, and Missouri).
AMH measurement was performed in the same laboratory using the same assay by one operator. The AMH serum levels were measured using an enzyme-linked immunosorbent assay (ELISA) using the AMH Gen II kit by Beckman Coulter, Inc (Brea, CA, USA).The measurement was realized by automated Labotech (Alka Technology®). The reading was performed at a wavelength at 450 nm. To calculate the AMH value, we used the point to point method. Values are presented in concentrations of ng/ml (convert to SI units: 1 ng/ ml = 7,14pM). The analytic sensibility was 0.008 ng/ml.
5
Hepatitis B Surface Antigen Detection
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Chemiluminescent microparticle immunoassays (CMIA) for the qualitative detection of hepatitis B surface antigen (HBsAg) in serum from the patients were performed using ARCHITECT HBsAg Qualitative II assay with ARCHITECT i system equipment (Abbot Laboratories, Illinois, USA). Serum of 125 microliter (μl) was used for each test. ARCHITECT HBsAg Qualitative II Controls (negative-and positive-controls) and Calibrators were used for quality control. The sample with the ratio of Sample Relative Light Unit/Cut-off Relative Light Unit (S/CO) <1.000 was interpreted as nonreactive (negative). The sample with the ratio of S/CO >1.000 was interpreted as reactive, which was then con rmed either if >100 S/CO by the Alere Determine™ HBsAg Test, a rapid in vitro qualitative immunoassay, (Abbot Laboratories, Illinois, USA), or if <100 S/CO by VIDAS® HBs Ag Ultra test, an Enzyme-Linked Fluorescent Assay (ELFA), (bioMérieux S.A., Marcy-l'Étoile, France). When the sample with S/CO >1.000 was reactive with either of the con rmatory tests, it was interpreted as HBsAg positive. The above strategy was based on WHO guidelines on hepatitis B and C testing. Geneva: World Health Organization; 2017. License: CC BY-NC-SA 3.0 IGO. ISBN: 978-92-4-154998-1) [27] .
6
Immunoglobulin Quantification in Chagas Serum
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Serial serum samples were conserved at -20°C in the Chagas serum bank in the Laboratory of Immunodiagnostic in INI/Fiocruz until use.
The quantification of total IgG, IgG1, IgG2, IgG3, IgG4, IgA, and IgM was performed by automatized Nephelometer ARRAY 360 system (Beckman and Coulter, USA) following manufacturer instructions. The immunoglobulin quantification was expressed as mg/dl, and a standard reference serum was used to calibrate the measurements. The total IgE was quantified by Enzyme-Linked Fluorescent Assay ELFA (Bio-Merieux, Brazil) in the VIDAS (Bio-Merieux) according to manufacturer instructions. The IgE quantification was expressed as international units (IU/L). Highly reactive samples were tested twice for results confirmation.
The quantification of total IgG, IgG1, IgG2, IgG3, IgG4, IgA, and IgM was performed by automatized Nephelometer ARRAY 360 system (Beckman and Coulter, USA) following manufacturer instructions. The immunoglobulin quantification was expressed as mg/dl, and a standard reference serum was used to calibrate the measurements. The total IgE was quantified by Enzyme-Linked Fluorescent Assay ELFA (Bio-Merieux, Brazil) in the VIDAS (Bio-Merieux) according to manufacturer instructions. The IgE quantification was expressed as international units (IU/L). Highly reactive samples were tested twice for results confirmation.
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