Tlc plates silica gel 60

Manufactured by Merck Group

Sourced in Germany

TLC plates silica gel 60 are thin-layer chromatography (TLC) plates made of silica gel. Silica gel is a porous, amorphous form of silicon dioxide that is commonly used as a stationary phase in TLC. The plates provide a substrate for the separation and analysis of chemical compounds.

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Lab products found in correlation

Silica gel 60, by Merck Group (2 mentions) Azithromycin, by Cayman Chemical (1 mentions) Hybri max, by Merck Group (1 mentions) Cholesterol d7, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

Silica gel 60 TLC plates Pre-coated silica gel 60 F254 TLC plates Precoated TLC plates (silica gel 60 F254) High-performance TLC silica gel 60 plates Aluminum silica gel 60 F254 TLC plates TLC silica gel 60 F254 TLC plates TLC silica gel 60 glass plates TLC silica gel 60 F 254 aluminum plates TLC silica gel 60 F254 glass plates TLC Silica gel 60 F254 20×20 cm glass plates

4 protocols using tlc plates silica gel 60

Chlamydia Lipid Metabolism Assay

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Azithromycin and N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-d-erythro-sphingosine (C₆-NBD-ceramide) were purchased from Cayman Chemical (Ann Arbor, MI, USA). In order to generate stock solutions, these compounds were dissolved in DMSO (the ultra-pure grade Hybri-Max™, Sigma-Aldrich, St. Louis, MO, USA) and were stored at −20 °C until use. l-[¹⁴C(U)]serine was obtained from Moravek (Brea, CA, USA). D7-cholesterol was purchased from Avanti Polar Lipids (Alabaster, AL, USA). FITC-conjugated mouse anti-chlamydial LPS antibody was purchased from Denka Seiken (Tokyo, Japan). DMEM with high glucose and cycloheximide was obtained from FUJIFILM Wako (Osaka, Japan). The TLC plates (Silica gel 60) were purchased from Merck (Darmstadt, Germany).

Kumagai K., Sakai S., Ueno M., Kataoka M., Kobayashi S, & Hanada K. (2022). Chlamydial Infection-Dependent Synthesis of Sphingomyelin as a Novel Anti-Chlamydial Target of Ceramide Mimetic Compounds. International Journal of Molecular Sciences, 23(23), 14697.

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Silica Gel Column Chromatography

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Column chromatography was performed by using Merck silica gel 60 (63–200 µm) with eluents indicated in the experimental section. Standard TLC for synthesis employed Merck TLC plates silica gel 60 on an aluminium base with the indicated solvent system. The spots on TLC were visualised either by UV–Vis (254 nm) or by staining with KMnO₄.

d’Orchymont F, & Holland J.P. (2023). Asymmetric rotaxanes as dual-modality supramolecular imaging agents for targeting cancer biomarkers. Communications Chemistry, 6, 107.

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Analytical Thin-Layer Chromatography

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Analytical thin-layer chromatography (TLC): Merck TLC plates (silica gel 60) on glass with the indicated solvent system. TLC spots were visualized by UV light (254 nm) and by stains of KMnO4 or ninhydrin. Flash column chromatography was performed using Merck silica gel 60 (40−63 μm particle size) with the indicated solvent system.

Duffet L., Tatarskiy P.V., Harada M., Williams E.T., Hartrampf N, & Patriarchi T. (2022). A photocaged orexin-B for spatiotemporally precise control of orexin signaling. Cell Chemical Biology, 29(12), 1729-1738.e8.

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Synthesis of Functionalized ssPalms

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All reagents were obtained from commercial sources and were used without further purification. Thin layer chromatography was performed on Merck TLC plates silica gel 60. ¹H-NMR spectra were recorded on a JEOL ECA600 (1H 600 MHz) spectrometer. Scheme for the synthesis of the ssPalmO and ssPalmO-Paz4-C2 is shown in Scheme 1. Intermediate Compounds 1 and 2 were synthesized as described in a previous report [5] (link). Compound 1 (1.2 g, 3.9 mmol) was dissolved in acetonitrile (31 mL) at 20–25 °C, and potassium carbonate (1.3 g, 9.7 mmol) was then added. After stirring the mixture at 20–25 °C for 5 minutes, 1-piperazineethanol (5.0 g, 39 mmol) was added and the resulting solution was stirred at 25–35 °C for 13 hours. The potassium carbonate was removed by filtration, and the filtrate was evaporated to give a brown liquid, which was then dissolved in chloroform (25 mL), and the chloroform phase washed with distillated water (3 × 25 mL). The organic layer was dried over magnesium sulfate (0.60 g), the solution was filtered and evaporated to yield compound 3 (1.0 g).Scheme 1

Syntheses of ssPalms. Scheme for the synthesis of the ssPalmO and ssPalmO-Paz4-C2 is shown. ssPalms were synthesized according to the Synthesis of the ssPalmO, ssPalmO-Paz4-C2, and ssPalmL section.

Scheme 1

Tanaka H., Watanabe A., Konishi M., Nakai Y., Yoshioka H., Ohkawara T., Takeda H., Harashima H, & Akita H. (2018). The delivery of mRNA to colon inflammatory lesions by lipid-nano-particles containing environmentally-sensitive lipid-like materials with oleic acid scaffolds. Heliyon, 4(12), e00959.

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