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Anti rabbit igg and anti rabbit fluorescein conjugated secondary antibody

Manufactured by Vector Laboratories
Sourced in Argentina, Germany

Anti-rabbit IgG and anti-rabbit fluorescein-conjugated secondary antibody are laboratory reagents used in immunological assays. The anti-rabbit IgG antibody binds to rabbit immunoglobulin G (IgG) proteins, while the fluorescein-conjugated version is labeled with a fluorescent dye to enable detection.

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2 protocols using anti rabbit igg and anti rabbit fluorescein conjugated secondary antibody

1

Apoptosis Induction Assay with Liposomal Doxorubicin

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Humanin peptide was obtained from Genemed Synthesis (San Antonio, TX). Culture media were obtained from Gibco (Invitrogen, Carlsbad, CA), fetal bovine serum (FBS) from Natocor (Buenos Aires, Argentina) and all terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) reagents from Roche Molecular Biochemicals (Mannheim, Germany). Liposomal doxorubicin hydrochloride (DOXO) was obtained from Dalmonar®, Tuteur (Argentina), and anti-rabbit IgG and anti-rabbit fluorescein-conjugated secondary antibody from Vector Laboratories Inc. (Burlingame, CA). Primers were obtained from Macrogen (Rockville, MD), Biodynamics (GenScript Inc, Piscataway, NJ) and Integrated DNA Technologies (Buenos Aires, Argentina) and the materials indicated below.
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2

Apoptosis Assay Protocol

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All chemicals and reagents were obtained from Sigma Aldrich. (St. Louis, MO) except for phenol red-free Dulbecco's modified Eagle's medium and supplements (D-MEM; GIBCO, Invitrogen, Carlsbad, CA), fetal bovine serum (Natocor, Buenos Aires, Argentina), all terminal deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) reagents (Roche Molecular Biochemicals, Mannheim, Germany), anti-rabbit IgG and anti-rabbit fluorescein-conjugated secondary antibody (Vector Laboratories Inc., Burlingame, CA), plasmids and primers (GenScript Inc, Piscataway, NJ), and the materials indicated below.
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