AMPKα (2532), phosphor-AMPKα (T172) (2531), ACC (3662) and phosphor-ACC (S79) (11818) antibodies were purchased from Cell Signaling Technology. UCP1 (ab23841) antibody was purchased from Abcam. β-actin (A5316) antibody was purchased from Sigma-Aldrich. Mouse IgG (NA931V) and rabbit IgG (NA934V) HRP-linked whole antibodies were purchased from GE Healthcare. To generate an anti-HDAC11 antibody, a peptide corresponding to amino acids 179–193 of HDAC11 (DLDAHQGNGHERDFM) was injected subcutaneously into New Zealand white rabbits (Alpha Diagnostic, Inc.). The resulting antibody was immune-affinity purified on a peptide column with Affi-gel agarose (Bio-Rad). Specificity of this anti-HDAC11 antibody was confirmed using brain tissue extracts from HDAC11 KO and WT mice. Oil red O (O0625) and oleic acid (O3008) were purchased from Sigma-Aldrich. AdipoRon (15941) was purchased from Cayman Chemical, and Insulin-Transferrin-Selenium (41400–045) was from Thermo Fisher Scientific.
Oleic acid o3008
Manufactured by Merck Group
Sourced in United States
Oleic acid (O3008) is a long-chain monounsaturated fatty acid that is commonly used in laboratory settings. It serves as a precursor for various chemical reactions and is often utilized in the synthesis of other compounds. The product specifications and technical details are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
β actin antibody a5316, by Merck Group (1 mentions)
Oil red o o0625, by Merck Group (1 mentions)
Affi gel agarose, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Penicillin streptomycin, by Cytiva (1 mentions)
Dulbecco s modified eagle medium dmem, by Cytiva (1 mentions)
H3k9me3, by Merck Group (1 mentions)
β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
2 protocols using oleic acid o3008
1
Antibodies and Reagents for AMPK Signaling
2
Adipogenesis Regulation by JMJD2B
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s modified Eagle medium (DMEM), penicillin–streptomycin, and fetal bovine serum (FBS) were obtained from HyClone Laboratories Inc. (Logan, UT, USA). The antibody against JMJD2B (NB100-74605) was purchased from Novus Biologicals (Littelton, CO, USA). Antibodies against H3K9me (07–450), H3K9me2 (07–441), and H3K9me3 (07–442) were obtained from Millipore (Billerica, MA, USA). Antibodies against PPARγ2 (sc-166731), and β-actin (sc-47778) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Sodium palmitate (P09767), bovine serum albumin (A6003) and oleic acid (O3008) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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