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Rat leptin elisa

Manufactured by BioVendor
Sourced in United States, Czechia

The Rat Leptin ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of leptin in rat serum, plasma, and other biological fluids. It utilizes a specific antibody coated on a microplate and a biotin-conjugated antibody for detection.

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2 protocols using rat leptin elisa

1

Plasma Biomarkers of Metabolic Health

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Plasma levels of insulin, leptin and adiponectin were measured using the following enzyme-linked immunosorbent assay (ELISA) kits: Rat Insulin ELISA (EZRMI-13K; Merck Millipore, Burlington, MA, United States), Rat Leptin ELISA (RD291001200R; BioVendor, Brno, Czech Republic) and Rat Adiponectin ELISA (EZRADP-62K; Merck Millipore, Burlington, MA, United States), according to the manufacturer’s instructions. Intra-assay variation coefficients were lower than 5% for all assays.
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2

Hormone and Adipokine Measurement

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Plasma levels of insulin and thyroid hormones (T3 and T4) were determined with a radioimmunoassay (RIA) using a commercial Insulin Rat 125I RIA Kit (DRG Instruments GmbH, Marburg, Germany) and T3 [125I] and T4 [125I] RIA Kits (Izotop, Budapest, Hungary), according to the manufacturer’s instructions. Plasma leptin and adiponectin levels were measured by an enzyme-linked immunosorbent assay (ELISA) using commercial Rat Leptin ELISA (BioVendor, Brno, Czech Republic) and Rat Adiponectin ELISA Kits (MilliporeSigma, Burlington, MA, USA). Intra-assay variation coefficients were lower than 5% for all assays.
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