Commercially available, lyophilized CA-II from bovine erythrocytes (Sigma) was used for all of the studies presented here, as purchased; all reagents were used, as purchased, without further purification. All buffer solutions were made with 18.1 MΩ water from a Barnstead NANOpure system. Metal-binding pharmacophores (MBPs) were prepared according to published procedures.(7 (link)) All MBP complexes for spectroscopy were prepared with 2 molar equivalents of the MBP, at the stated concentrations.
Ca 2 from bovine erythrocytes
Manufactured by Merck Group
CA-II from bovine erythrocytes is a laboratory product that functions as a source of carbonic anhydrase II, an enzyme found in bovine red blood cells. This enzyme plays a role in the reversible conversion of carbon dioxide and water to carbonic acid, bicarbonate, and protons. The product is intended for use in various research and analytical applications that require carbonic anhydrase II.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin hrp, by BioLegend (1 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (1 mentions)
Powercho, by Lonza (1 mentions)
Oligonucleotides, by Biosearch Technologies (1 mentions)
Ultraglutamine, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
Tween 20, by Merck Group (1 mentions)
Biacore s200, by Cytiva (1 mentions)
2 protocols using ca 2 from bovine erythrocytes
1
Standardized Bovine CA-II Protocols
2
Biochemical Reagent and Equipment Protocols
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Reagents. Starting materials, solvents, and reagents, unless otherwise noted, were used as supplied by Fisher Chemicals, Merck, VWR or Sigma-Aldrich in HPLC or analytical grade. All other reagents (carboxylic acids, isothiocyanates) were purchased from several commercial suppliers including Enamine, ABCR and Sigma Aldrich. Oligonucleotides were purchased from LGC Biosearch Technologies. Power-CHO and Pro-CHO medium for cell culturing were purchased from Lonza and supplied with 8 M Ultraglutamine (Gibco), 4 M HT (Gibco), and 1% antibiotic-antimycotic (Gibco). CAII from bovine erythrocytes was purchased from Sigma-Aldrich (Cat. n. C2624). Monoclonal Anti-His-HRP, streptavidin-HRP and proteinA-HRP protein conjugates were purchased from BioLegend. A pre-made solution of 3,3',5,5'-tetramethylbenzidine (TMB) was purchased from Sigma-Aldrich (Cat. n. T4444). Tween 20 was purchased from Sigma-Aldrich (P6585). SPR measurements were performed on a BIAcore S200 (Cytiva, formerly GE Healthcare Life Sciences). CM5 BIAcore microsensor chips and other BIAcore associated reagents (e.g., HPS-EP buffer, vials, etc.) were purchased from Cytiva.
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