Rabbit anti tyrosine hydroxylase anti th polyclonal primary antibody

The required chemicals viz., Rotenone (Sigma, Cat: R8875), Curcumin (Sigma, Cat: 1386) and DMSO (Sigma, Cat: D8418) were used for feeding procedures. Standard dopamine (DA; Sigma-Aldrich, Cat: H8502) and its metabolites 3,4-Dihydroxyphenylacetic acid (DOPAC; Sigma-Aldrich, Cat: 11569) and Homovanilic acid (HVA; Sigma-Aldrich, Cat: 69673) were used for quantifying DA and metabolites. Phosphate-buffered Saline (PBS; HiMedia, India, Cat: ML023), Potassium ferricyanide (KCN; HiMedia, India, Cat: GRM627), and Nicotinamide adenine dinucleotide (NADH; SRL. India, Cat: 44018) were used for quantifying mitochondrial complex I activity. Paraformaldehyde (Sigma, Cat: I58127), Triton X-100 (Sigma, Cat: T8787), Normal Goat Serum (NGS; Vector Lab, Cat: S1000), VECTASHIELD mounting medium (Vector Labs, CA, United States, Cat: H1000), Rabbit anti-Tyrosine hydroxylase (anti-TH) polyclonal primary antibody (Millipore, MA, United States, Cat: Ab152), and Goat anti-rabbit IgG H&L (TRITC labeled) polyclonal secondary antibody (Abcam, MA, United States, Cat: Ab6718) were used for immunostaining.

The required chemicals viz., Methyl viologen dichloride hydrate/Paraquat (PQ) (Sigma-Aldrich; St. Louis, MO, USA, Cat. No. 856177), Sucrose (SRL, Maharashtra, India, Cat. No. 1947139), DMSO (Sigma-Aldrich; St. Louis, MO, USA, Cat. No. D8418). Phosphate buffered Saline (PBS; HiMedia, Maharashtra, India, Cat. No. ML023), Trichloro Acetic Acid (TCA; SRL, Maharashtra, India, Cat. No. 204842), Dopamine (DA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. H8502); 3,4-Dihydroxyphenylacetic acid (DOPAC; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. 11569); Homovanillic acid (HVA; Sigma-Aldrich, St. Louis, MO, USA, Cat. No. 69673) were used for quantifying DA and metabolites. Paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA, Cat. No. I58127), Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA, Cat. No. T8787), Triton X-100 (Sigma, St. Louis, MO, USA), Normal goat serum (NGS, Vector labs, CA, USA), VECTASHIELD mounting medium (Vector Labs, CA, USA, Cat: H1000), Rabbit anti-Tyrosine hydroxylase (anti-TH) polyclonal primary antibody (Millipore, MA, USA, Cat: Ab152) and Goat anti-rabbit IgG H&L (TRITC labeled) polyclonal secondary antibody (Abcam, MA, USA, Cat: Ab6718) were used for immunostaining. Whatman filter paper no. 1 disk was used as a feeding medium in the experiment in a 30 × 100 mm glass vial.