Chitosan cts

Chemicals including pyridine, 2-aminopyridine (2-AP), aniline, 2-amino-5-chloropyridine (2-A-5-CP), phenylenediamine, maleic anhydride (MAH), oleic acid were purchased from TCI Development Co., Ltd. (Shanghai, China). Monomer methacrylic acid (MAA) and ethyleneglycol dimethacrylate (EGDMA) were obtained from Sigma-Aldrich. 2, 2’-azobis (2-methylpropionitrile) (AIBN, initiator) was purchased from J&K Technology Limited (Beijing, China). FeCl₃·6H₂O, FeCl₂·4H₂O, polyvinylpyrrolidone (PVP), ammonium hydroxide (NH₃·H₂O) and chitosan (CTs) with 95% deacetylation and an average molecular weight of 1 × 10⁵ g·mol⁻¹ were obtained from Sinopharm Chemical Reagent (Shanghai, China). β-cyclodextrin (β-CD) was supplied by TCI and recrystallized for purification. N,N-Dimethylformamide, acetonitrile, chloroform, toluene, acetic acid and other reagents were AR or HPLC grade and were supplied by Fisher Scientific (Shanghai, China).

Chitosan (CTS) and silver nitrate (AgNO₃) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Trisodium citrate (TSC), polyvinyl alcohol (PVA), bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT), and sodium borohydride (NaBH₄) were purchased from Aladdin Chemical Reagent Co., Ltd., (Shanghai, China). N, N′-bisacrylamide cystamine (BAC) was purchased from Alfa Aesar Chemical Co., Ltd. (Shanghai, China). Doxorubicin (DOX) was purchased from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China). CAL27 cells (human oral squamous cell carcinoma cell line, OSCC) were derived from the First Affiliated Hospital of Nanchang University (Nanchang, China). Dulbecco-modified eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from GIBCO Co., Ltd. (California, United States). Unless otherwise stated, the other chemical reagents were purchased from the Chinese Pharmaceutical Chemical Reagent Co., Ltd. (Shanghai, China), and the biological reagents were bought from Sigma-Aldrich (MO, United States). The abbreviations used in this article are summarized as follows: Ag-BAC (bAG), CTS/Ag-BAC (CAB), and CTS/Ag-BAC@DOX (CAB@DOX).