Mircury lna knockdown probes

Manufactured by Qiagen

The MiRCURY LNA knockdown probes are a set of oligonucleotide-based reagents designed for the selective inhibition of microRNA (miRNA) expression in cells and tissues. These probes utilize Locked Nucleic Acid (LNA) technology to enhance their affinity and specificity towards target miRNAs, enabling efficient knockdown of miRNA activity.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Dharmafect 1 df1, by Horizon Discovery (1 mentions)

Spelling variants of this product

LNA probes (48 citations) MiRCURY LNA probes (19 citations) LNA miRCuRY (2 citations)

2 protocols using mircury lna knockdown probes

Generating Neurospheres via miRNA Knockdown

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hiPSC-derived NPCs were transiently transfected with miRCURY LNA knockdown probes (Exiqon) with Lipofectamine RNAiMAX (Life Technology) in Optimem (ThermoFisher Scientific) for 24 hours. A scrambled miRNA, which bears no homology to any known miRNA or mRNA sequences in human, mouse, and rat, was used as negative control, and hsa-anti-miR-9 (410014-08) (100 nM) was used to specifically inhibit miR-9. 24 hours after transfection, NPCs were dissociated with accutase in order to generate neurospheres. Neurospheres were additionally cultured in miRCURY LNA knockdown probes (Exiqon) with Lipofectamine RNAiMAX (Life Technology) in NPC media.

Topol A., Zhu S., Hartley B.J., English J., Hauberg M.E., Readhead B., Tran N., Rittenhouse C.A., Simone A., Ruderfer D.M., Johnson J., Hadas Y., Gochman P.A., Wang Y.C., Shah H., Cagney G., Rapoport J., Gage F.H., Dudley J.T., Sklar P., Mattheisen M., Cotter D., Fang G, & Brennand K.J. (2016). Dysregulation of miRNA-9 in a subset of schizophrenia patient-derived neural progenitor cells. Cell reports, 15(5), 1024-1036.

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miRNA-155 Knockdown in Macrophages

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Both miR-155-5p and miR-155-3p were inhibited using miRCURY LNA knockdown probes (Exiqon; miR-155-5p, #410078-00; miR-155-3p, #410079-00; negative control Scramble-miR, #199002-00). Oligonucleotides were transfected into macrophages using Dharmafect 1 (DF1, Dharmacon) using a well-optimised method
^{32 (link)}. Briefly, complexes of oligonucleotides in a 1/50 dilution of DF1 in OmtiMEM (Life Technologies) were prepared. Transfections took place in serum-free RPMI with no phenol red to which complexes were added in a final proportion of 75:25%. The recovery time between transfection and stimulation was 3.5 hrs. Pilot experiments showed that allowing cells to recover overnight (18hrs) resulted in loss of miRNA inhibition. After recovery, media was replaced with complete RPMI, then stimulated with LPS for 2 hrs.

, & Simmonds R.E. (2019). Transient up-regulation of miR-155-3p by lipopolysaccharide in primary human monocyte-derived macrophages results in RISC incorporation but does not alter TNF expression. Wellcome Open Research, 4, 43.

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